Key Product Details

Species Reactivity

Human, Canine, Monkey, Primate

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2C8

Format

Azide and BSA Free
View all SSB Primary Antibodies »

Product Specifications

Immunogen

Full-length protein expressed in 293T cell transfected with human SSB expression vector

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

46.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SSB Antibody (OTI2C8) - Azide and BSA Free

Western Blot: SSB Antibody (OTI2C8)Azide and BSA Free [NBP2-74356]

Western Blot: SSB Antibody (OTI2C8)Azide and BSA Free [NBP2-74356]

Western Blot: SSB Antibody (OTI2C8) - Azide and BSA Free [NBP2-74356] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SSB (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SSB.
Immunocytochemistry/ Immunofluorescence: SSB Antibody (OTI2C8) - Azide and BSA Free [NBP2-74356]

Immunocytochemistry/ Immunofluorescence: SSB Antibody (OTI2C8) - Azide and BSA Free [NBP2-74356]

Immunocytochemistry/Immunofluorescence: SSB Antibody (OTI2C8) - Azide and BSA Free [NBP2-74356] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY SSB.
Immunohistochemistry: SSB Antibody (OTI2C8) - Azide and BSA Free [NBP2-74356]

Immunohistochemistry: SSB Antibody (OTI2C8) - Azide and BSA Free [NBP2-74356]

Immunohistochemistry: SSB Antibody (OTI2C8) - Azide and BSA Free [NBP2-74356] - Staining of paraffin-embedded Human prostate tissue using anti-SSB mouse monoclonal antibody.
Western Blot: SSB Antibody (OTI2C8)Azide and BSA Free [NBP2-74356]

Western Blot: SSB Antibody (OTI2C8)Azide and BSA Free [NBP2-74356]

Western Blot: SSB Antibody (OTI2C8) - Azide and BSA Free [NBP2-74356] - Analysis of extracts (35ug) from 9 different cell lines by using anti-SSB monoclonal antibody.
Immunohistochemistry: SSB Antibody (OTI2C8) - Azide and BSA Free [NBP2-74356]

Immunohistochemistry: SSB Antibody (OTI2C8) - Azide and BSA Free [NBP2-74356]

Immunohistochemistry: SSB Antibody (OTI2C8) - Azide and BSA Free [NBP2-74356] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-SSB mouse monoclonal antibody.
Immunohistochemistry: SSB Antibody (OTI2C8) - Azide and BSA Free [NBP2-74356]

Immunohistochemistry: SSB Antibody (OTI2C8) - Azide and BSA Free [NBP2-74356]

Immunohistochemistry: SSB Antibody (OTI2C8) - Azide and BSA Free [NBP2-74356] - Staining of paraffin-embedded Carcinoma of Human lung tissue using anti-SSB mouse monoclonal antibody.
Immunohistochemistry: SSB Antibody (OTI2C8) - Azide and BSA Free [NBP2-74356]

Immunohistochemistry: SSB Antibody (OTI2C8) - Azide and BSA Free [NBP2-74356]

Immunohistochemistry: SSB Antibody (OTI2C8) - Azide and BSA Free [NBP2-74356] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-SSB mouse monoclonal antibody.
Immunohistochemistry: SSB Antibody (OTI2C8) - Azide and BSA Free [NBP2-74356]

Immunohistochemistry: SSB Antibody (OTI2C8) - Azide and BSA Free [NBP2-74356]

Immunohistochemistry: SSB Antibody (OTI2C8) - Azide and BSA Free [NBP2-74356] - Staining of paraffin-embedded Human breast tissue using anti-SSB mouse monoclonal antibody.
Immunohistochemistry: SSB Antibody (OTI2C8) - Azide and BSA Free [NBP2-74356]

Immunohistochemistry: SSB Antibody (OTI2C8) - Azide and BSA Free [NBP2-74356]

Immunohistochemistry: SSB Antibody (OTI2C8) - Azide and BSA Free [NBP2-74356] - Staining of paraffin-embedded Human colon tissue using anti-SSB mouse monoclonal antibody.
Immunohistochemistry: SSB Antibody (OTI2C8) - Azide and BSA Free [NBP2-74356]

Immunohistochemistry: SSB Antibody (OTI2C8) - Azide and BSA Free [NBP2-74356]

Immunohistochemistry: SSB Antibody (OTI2C8) - Azide and BSA Free [NBP2-74356] - Staining of paraffin-embedded Human endometrium tissue using anti-SSB mouse monoclonal antibody.
Immunohistochemistry: SSB Antibody (OTI2C8) - Azide and BSA Free [NBP2-74356]

Immunohistochemistry: SSB Antibody (OTI2C8) - Azide and BSA Free [NBP2-74356]

Immunohistochemistry: SSB Antibody (OTI2C8) - Azide and BSA Free [NBP2-74356] - Staining of paraffin-embedded Human Kidney tissue using anti-SSB mouse monoclonal antibody.
Immunohistochemistry: SSB Antibody (OTI2C8) - Azide and BSA Free [NBP2-74356]

Immunohistochemistry: SSB Antibody (OTI2C8) - Azide and BSA Free [NBP2-74356]

Immunohistochemistry: SSB Antibody (OTI2C8) - Azide and BSA Free [NBP2-74356] - Staining of paraffin-embedded Human lung tissue using anti-SSB mouse monoclonal antibody.
Immunohistochemistry: SSB Antibody (OTI2C8) - Azide and BSA Free [NBP2-74356]

Immunohistochemistry: SSB Antibody (OTI2C8) - Azide and BSA Free [NBP2-74356]

Immunohistochemistry: SSB Antibody (OTI2C8) - Azide and BSA Free [NBP2-74356] - Staining of paraffin-embedded Human Ovary tissue using anti-SSB mouse monoclonal antibody.
Immunohistochemistry: SSB Antibody (OTI2C8) - Azide and BSA Free [NBP2-74356]

Immunohistochemistry: SSB Antibody (OTI2C8) - Azide and BSA Free [NBP2-74356]

Immunohistochemistry: SSB Antibody (OTI2C8) - Azide and BSA Free [NBP2-74356] - Staining of paraffin-embedded Human pancreas tissue using anti-SSB mouse monoclonal antibody.
Immunoprecipitation: SSB Antibody (OTI2C8) - Azide and BSA Free [NBP2-74356]

Immunoprecipitation: SSB Antibody (OTI2C8) - Azide and BSA Free [NBP2-74356]

Immunoprecipitation: SSB Antibody (OTI2C8) - Azide and BSA Free [NBP2-74356] - (Negative control: IP without adding anti-SSB antibody.). For each experiment, 500ul of DDK tagged SSB overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-SSB antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

Applications for SSB Antibody (OTI2C8) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunoprecipitation

2ug/500ul

Western Blot

1:1000-2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SSB

Members of the suppressor of cytokine signaling (SOCS) family of proteins contain C-terminal regions of homology called the SOCS box, which serves to couple SOCS proteins and their binding partners with the Elongin B and C complex, thereby mediating protein degradation. Several other families of proteins also contain SOCS boxes, but differ from the SOCS proteins in the type of domain they contain upstream of the SOCS box. SSB-2 (SplA/ryanodine (SPRY) receptor domain-containing SOCS box protein 2), also known as GGRCC9 (gene-rich cluster protein C9), SPSB2 or MGC2519, is a cytoplasmic protein belonging to the SPSB family of proteins that contain a central SPRY domain and a C-terminal SOCS box. The SPRY domain is believed to be a protein-protein interaction motif. Members of the SPSB family are capable of interacting with Met (a receptor for hepatocyte growth factors (HGFs)), and SSB-1 is known to promote HGF signaling. SSB-1, SSB-2 and SSB-4 are also able to interact with PAR4 (prostate apoptosis response protein 4). Mutations in the genes encoding SPSB proteins are associated with several human diseases.

Alternate Names

La autoantigen, La ribonucleoprotein, lupus La protein, member 3, Sjoegren syndrome type B antigen, Sjogren syndrome antigen B (autoantigen La), SS-B, SS-B/La protein

Gene Symbol

SSB

Additional SSB Products

Product Documents for SSB Antibody (OTI2C8) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for SSB Antibody (OTI2C8) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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