SSB Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-35080

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all SSB Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 166-408 of human SSB (NP_003133.1).

Sequence:
KFVETPGQKYKETDLLILFKDDYFAKKNEERKQNKVEAKLRAKQEQEAKQKLEEDAEMKSLEEKIGCLLKFSGDLDDQTCREDLHILFSNHGEIKWIDFVRGAKEGIILFKEKAKEALGKAKDANNGNLQLRNKEVTWEVLEGEVEKEALKKIIEDQQESLNKWKSKGRRFKGKGKGNKAAQPGSGKGKVQFQGKKTKFASDDEHDEHDENGATGPVKRAREETDKEEPASKQQKTENGAGDQ

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SSB Antibody - BSA Free

SSB Antibody

Immunohistochemistry: SSB Antibody [NBP3-35080] -

Immunohistochemistry: SSB Antibody [NBP3-35080] - Immunohistochemistry analysis of paraffin-embedded Human lung cancer using SSB Rabbit pAb at dilution of 1:300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
SSB Antibody

Immunoprecipitation: SSB Antibody [NBP3-35080] -

Immunoprecipitation: SSB Antibody [NBP3-35080] - Immunoprecipitation analysis of 200 ug extracts of Jurkat cells using 3 ug SSB antibody. Western blot was performed from the immunoprecipitate using SSB antibody at a dilution of 1:1000.
SSB Antibody

Immunohistochemistry: SSB Antibody [NBP3-35080] -

Immunohistochemistry: SSB Antibody [NBP3-35080] - Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using SSB Rabbit pAb at dilution of 1:300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
SSB Antibody

Immunohistochemistry: SSB Antibody [NBP3-35080] -

Immunohistochemistry: SSB Antibody [NBP3-35080] - Immunohistochemistry analysis of paraffin-embedded Human breast cancer using SSB Rabbit pAb at dilution of 1:300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
SSB Antibody

Immunohistochemistry: SSB Antibody [NBP3-35080] -

Immunohistochemistry: SSB Antibody [NBP3-35080] - Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer using SSB Rabbit pAb at dilution of 1:300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
SSB Antibody

Immunocytochemistry/ Immunofluorescence: SSB Antibody [NBP3-35080] -

Immunocytochemistry/ Immunofluorescence: SSB Antibody [NBP3-35080] - Immunofluorescence analysis of U2OS cells using SSB Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
SSB Antibody

Western Blot: SSB Antibody [NBP3-35080] -

Western Blot: SSB Antibody [NBP3-35080] - Western blot analysis of various lysates using SSB Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.

Applications for SSB Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry-Paraffin

1:100 - 1:500

Immunoprecipitation

0.5ug - 4ug antibody for 200ug - 400ug extracts of whole cells

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: SSB

Members of the suppressor of cytokine signaling (SOCS) family of proteins contain C-terminal regions of homology called the SOCS box, which serves to couple SOCS proteins and their binding partners with the Elongin B and C complex, thereby mediating protein degradation. Several other families of proteins also contain SOCS boxes, but differ from the SOCS proteins in the type of domain they contain upstream of the SOCS box. SSB-2 (SplA/ryanodine (SPRY) receptor domain-containing SOCS box protein 2), also known as GGRCC9 (gene-rich cluster protein C9), SPSB2 or MGC2519, is a cytoplasmic protein belonging to the SPSB family of proteins that contain a central SPRY domain and a C-terminal SOCS box. The SPRY domain is believed to be a protein-protein interaction motif. Members of the SPSB family are capable of interacting with Met (a receptor for hepatocyte growth factors (HGFs)), and SSB-1 is known to promote HGF signaling. SSB-1, SSB-2 and SSB-4 are also able to interact with PAR4 (prostate apoptosis response protein 4). Mutations in the genes encoding SPSB proteins are associated with several human diseases.

Alternate Names

La autoantigen, La ribonucleoprotein, lupus La protein, member 3, Sjoegren syndrome type B antigen, Sjogren syndrome antigen B (autoantigen La), SS-B, SS-B/La protein

Gene Symbol

SSB

Additional SSB Products

Product Documents for SSB Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for SSB Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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