Human ST2/IL-33R ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP2-60069

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and tissue homogenates (50 - 100 uL)

Sensitivity

0.078 ng/mL

Assay Range

0.312 ng/mL - 20 ng/mL
View all ST2/IL-33R ELISA Kits »

Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Human

Specificity

This assay has high sensitivity and excellent specificity for detection of human IL1RL1. No significant cross-reactivity or interference between human IL1RL1 and analogues was observed.

Description

Assay Length: 1-5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV 8

Inter-Assay Precision (Precision between assays) %CV 10

Scientific Data Images for Human ST2/IL-33R ELISA Kit (Colorimetric)

ELISA: Human ST2/IL-33R ELISA Kit (Colorimetric) [NBP2-60069]

ELISA: Human ST2/IL-33R ELISA Kit (Colorimetric) [NBP2-60069]

ELISA: Human IL1RL1 ELISA Kit (Colorimetric) [NBP2-60069] - These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Human ST2/IL-33R ELISA Kit (Colorimetric)

Human ST2/IL-33R ELISA Kit (Colorimetric) [NBP2-60069] -

Human ST2/IL-33R ELISA Kit (Colorimetric) [NBP2-60069] - Standard Curve Reference

Kit Contents for Human ST2/IL-33R ELISA Kit (Colorimetric)

  • Adhesive Strip (For 96 wells)
  • Assay plate (12 x 8 coated Microwells)
  • Biotin-antibody (100 x concentrate)
  • Biotin-antibody Diluent
  • HRP-avidin (100 x concentrate)
  • HRP-avidin Diluent
  • Instruction manual
  • Sample Diluent
  • Standard (Freeze dried)
  • Stop Solution
  • TMB Substrate
  • Wash Buffer (25 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: ST2/IL-33R

This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IL1RL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL1RL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL1RL1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL1RL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Long Name

Interleukin 33 Receptor

Alternate Names

DER4, Fit-1, IL-1 R4, IL-1R4, IL-1RL1, IL-33 R, IL-33R, IL1R4, IL33R, Ly84, ST2L, ST2V, T1

Gene Symbol

IL1RL1

Additional ST2/IL-33R Products

Product Documents for Human ST2/IL-33R ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Human ST2/IL-33R ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Related Research Areas

Group 2 ILCs
IL-1 Family

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