STING/TMEM173 Antibody (STING1/8129R) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-20698

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # STING1/8129R

Format

Azide and BSA Free
View all STING/TMEM173 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fragment (around aa190-290) of human STING/TMEM173 protein (exact sequence is proprietary)

Localization

Cytoplasm.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Description

Positive Controls: Cytoplasmic expression in several tissues including cells in respiratory tract, fallopian tube and cells in lymphoid tissues.

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.

Scientific Data Images for STING/TMEM173 Antibody (STING1/8129R) - Azide and BSA Free

STING/TMEM173 Antibody (STING1/8129R) - Azide and BSA Free

STING/TMEM173 Antibody (STING1/8129R)

Formalin-fixed, paraffin-embedded human mammary cancer stained with STING/TMEM173 antibody (STING1/8129R). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.
STING/TMEM173 Antibody (STING1/8129R) - Azide and BSA Free

STING/TMEM173 Antibody (STING1/8129R)

Formalin-fixed, paraffin-embedded human serous ovarian carcinoma stained with STING/TMEM173 antibody (STING1/8129R). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.
STING/TMEM173 Antibody (STING1/8129R) - Azide and BSA Free

STING/TMEM173 Antibody (STING1/8129R)

Formalin-fixed, paraffin-embedded human renal cell carcinoma stained with STING/TMEM173 antibody (STING1/8129R). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.
STING/TMEM173 Antibody (STING1/8129R) - Azide and BSA Free

STING/TMEM173 Antibody (STING1/8129R)

Formalin-fixed, paraffin-embedded human prostate carcinoma stained with STING/TMEM173 antibody (STING1/8129R). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.
STING/TMEM173 Antibody (STING1/8129R) - Azide and BSA Free

STING/TMEM173 Antibody (STING1/8129R)

Formalin-fixed, paraffin-embedded human spleen stained with STING/TMEM173 antibody (STING1/8129R). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.
STING/TMEM173 Antibody (STING1/8129R) - Azide and BSA Free

STING/TMEM173 Antibody (STING1/8129R)

Formalin-fixed, paraffin-embedded human tonsil stained with STING/TMEM173 antibody (STING1/8129R). Inset: PBS instead of primary antibody; secondary only negative control.

Applications for STING/TMEM173 Antibody (STING1/8129R) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.

Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: STING/TMEM173

STING (stimulator of interferon genes) is encoded by the TMEM173 gene and is an adaptor molecule involved in the activation of innate immune responses to PAMPS (pathogen-associated molecular patterns) and DAMPS (damage-associated molecular patterns). STING specifically recognizes cytosolic DNA products derived from pathogens (e.g., cytomegalovirus, vaccinia virus, Listeria monocytogenes) or dead cells (1, 2). In the STING pathway, dsDNA derived from pathogens or damaged cells serves as a substrate for the enzyme cGAS (cyclic GMP-AMP synthase) which produces the second messenger cyclic GMP-AMP (cGAMP) from ATP and GTP (3, 4). Under steady-state conditions STING (theoretical molecular weight 42 kDa), a protein localizes to the ER membrane. Upon activation by dsDNA derived second messenger (cGAMP), STING translocates to the Golgi apparatus as a homodimer. Once STING has trafficked to the perinuclear region, it activates TANK binding kinase 1 (TBK1), interferon regulatory factor 3 (IRF3) and NF-kB leading to the production of cytokines (e.g., type I interferon) (2, 4). Mutations in the TMEM173 gene affecting STING expression are associated with the development of the auto-inflammatory disease SAVI (STING-associated vasculopathy with onset in infancy) (2). A novel SAVI dominant mutation in the TMEM173 human gene (V155M) leads to increased localization of STING to the Golgi and perinuclear region, indicative of an activated state (1). Hallmarks of SAVI, a rare inflammatory disease, include severe vasculitis in extremities and lung inflammation (7).

References

1. Patel, S., & Jin, L. (2019). TMEM173 variants and potential importance to human biology and disease. Genes and Immunity. https://doi.org/10.1038/s41435-018-0029-9

2. Jounai, N., Kobiyama, K., Takeshita, F., & Ishii, K. J. (2013). Recognition of damage-associated molecular patterns related to nucleic acids during inflammation and vaccination. Frontiers in Cellular and Infection Microbiology. https://doi.org/10.3389/fcimb.2012.00168

3. Xiao, T. S., & Fitzgerald, K. A. (2013). The cGAS-STING Pathway for DNA Sensing. Molecular Cell. https://doi.org/10.1016/j.molcel.2013.07.004

4. Kato, K., Omura, H., Ishitani, R., & Nureki, O. (2017). Cyclic GMP-AMP as an Endogenous Second Messenger in Innate Immune Signaling by Cytosolic DNA. Annual Review of Biochemistry. https://doi.org/10.1146/annurev-biochem-061516-044813

5. Crowl, J. T., Gray, E. E., Pestal, K., Volkman, H. E., & Stetson, D. B. (2017). Intracellular Nucleic Acid Detection in Autoimmunity. Annual Review of Immunology. https://doi.org/10.1146/annurev-immunol-051116-052331

Long Name

Stimulator of Interferon Genes Protein/Transmembrane protein 173

Alternate Names

ERIS, MITA, MPYS, NET23, TMEM173

Gene Symbol

STING1

Additional STING/TMEM173 Products

Product Documents for STING/TMEM173 Antibody (STING1/8129R) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for STING/TMEM173 Antibody (STING1/8129R) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for STING/TMEM173 Antibody (STING1/8129R) - Azide and BSA Free

There are currently no reviews for this product. Be the first to review STING/TMEM173 Antibody (STING1/8129R) - Azide and BSA Free and earn rewards!

Have you used STING/TMEM173 Antibody (STING1/8129R) - Azide and BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies