Syntaxin 4 Antibody (8F3P7)

Novus Biologicals | Catalog # NBP3-16629

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 8F3P7 expressed in HEK293
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Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 50-150 of human Syntaxin 4 (NP_004595.2)). QTIVKLGNKVQELEKQQVTILATPLPEESMKQELQNLRDEIKQLGREIRLQLKAIEPQKEEADENYNSVNTRMRKTQHGVLSQQFVELINKCNSMQSEYRE

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit Syntaxin 4 Antibody (8F3P7) (NBP3-16629) is a recombinant monoclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Syntaxin 4 Antibody (8F3P7)

Western Blot: Syntaxin 4 Antibody (8F3P7) [NBP3-16629]

Western Blot: Syntaxin 4 Antibody (8F3P7) [NBP3-16629]

Western Blot: Syntaxin 4 Antibody (8F3P7) [NBP3-16629] - Western blot analysis of extracts of various cell lines, using Syntaxin 4 Rabbit mAb (NBP3-16629) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Western Blot: Syntaxin 4 Antibody (8F3P7) [NBP3-16629]

Western Blot: Syntaxin 4 Antibody (8F3P7) [NBP3-16629]

Western Blot: Syntaxin 4 Antibody (8F3P7) [NBP3-16629] - Western blot analysis of extracts of Rat brain, using Syntaxin 4 Rabbit mAb (NBP3-16629) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunocytochemistry/ Immunofluorescence: Syntaxin 4 Antibody (8F3P7) [NBP3-16629]

Immunocytochemistry/ Immunofluorescence: Syntaxin 4 Antibody (8F3P7) [NBP3-16629]

Immunocytochemistry/Immunofluorescence: Syntaxin 4 Antibody (8F3P7) [NBP3-16629] - Immunofluorescence analysis of NIH-3T3 cells using Syntaxin 4 Rabbit mAb (NBP3-16629) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Applications for Syntaxin 4 Antibody (8F3P7)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Syntaxin 4

Plasma membrane t-SNARE that mediates docking of transport vesicles. Necessary for the translocation of SLC2A4 from intracellular vesicles to the plasma membrane. Together with STXB3 and VAMP2, may also play a role in docking/fusion of intracellular GLUT4-containing vesicles with the cell surface in adipocytes. May also play a role in docking of synaptic vesicles at presynaptic active zones

Alternate Names

NY-REN-31, p35-2, STX4

Gene Symbol

STX4

Additional Syntaxin 4 Products

Product Documents for Syntaxin 4 Antibody (8F3P7)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Syntaxin 4 Antibody (8F3P7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

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Protocols

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