T-bet/TBX21 Antibody (4B10) - BSA Free

Novus Biologicals | Catalog # NBP1-43298

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rhesus Macaque

Applications

Western Blot, Flow Cytometry, Flow (Intracellular), Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 4B10

Format

BSA Free
View all T-bet/TBX21 Primary Antibodies »

Product Specifications

Immunogen

The immunogen for this antibody was TBet.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for T-bet/TBX21 Antibody (4B10) - BSA Free

Western Blot: T-bet/TBX21 Antibody (4B10)BSA Free [NBP1-43298]

Western Blot: T-bet/TBX21 Antibody (4B10)BSA Free [NBP1-43298]

Western Blot: T-bet/TBX21 Antibody (4B10) [NBP1-43298] - CD4+ T cells were sorted from mouse spleen, activated with Anti-Mouse CD3 and Anti-Mouse CD28, followed by culture in Th1-polarizing conditions, and re-activation with PMA and Ionomycin. Lysates from control (left) or PMA and Ionomycin-re-activated (right
Flow (Intracellular): T-bet/TBX21 Antibody (4B10) - BSA Free [NBP1-43298]

Flow (Intracellular): T-bet/TBX21 Antibody (4B10) - BSA Free [NBP1-43298]

Flow (Intracellular): T-bet/TBX21 Antibody (4B10) [NBP1-43298] - Intracellular staining of normal human peripheral blood cells with Anti-Human CD8a APC and 0.125 ug of Anti-Human/Mouse T-bet PerCP-Cy5.5 using the Foxp3 Fixation/Permeabilization Buffers. Cells in the lymphocyte gate were used for analysis. Image using the PerCP/Cy5.5 format of this antibody.

Applications for T-bet/TBX21 Antibody (4B10) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Immunoprecipitation

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
This 4B10 antibody has been tested by western blot analysis of Th1-polarized mouse CD4+ T cells and can be used at 2 ug/ml as a starting dilution for western blotting.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS (pH 7.2)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: T-bet/TBX21

The 4B10 monoclonal antibody reacts with mouse and human T-bet. T-bet is a Th1-specific T-box transcription factor critical to the development of the Th1 CD4+ T cell lineage. This is known based on the observations that T-Bet deficient mice have impaired Th1 cell development, and that ectopic expression of T-Bet results in development skewed to the Th1 lineage. T-Bet expression is induced by the Th1 cytokine IFN-gamma, and T-Bet also regulates the expression of IFN-gamma, likely, at least in part, through the modification of DNA accessibility and histone remodeling. In addition to IFN-gamma, T-Bet is also known to regulate the expression of IL-12Rbeta and IL-2. Moreover, T-Bet plays a role in class-switch recombination in B-cells.

Long Name

T-box transcription factor TBX21

Alternate Names

T-PET, Tbet, TBLYM, TBX21

Gene Symbol

TBX21

Additional T-bet/TBX21 Products

Product Documents for T-bet/TBX21 Antibody (4B10) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for T-bet/TBX21 Antibody (4B10) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs for T-bet/TBX21 Antibody (4B10) - BSA Free

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  • Q: Do you make a Tbx21 ab for FACS for porcine?

    A:

    We have not tested our Tbx21 antibodies for porcine, however you might like to test NBP1-43298 as part of our Innovators Reward Program. This is gained from testing a product on a species or application that we have not yet tested. I have carried out a blast to check the % homology between the pig protein and human protein, this is 94% and our lab recommends use if the % homology is 85% or above.

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