TAAR4 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-10140

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Rat

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit

Format

BSA Free
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Product Specifications

Immunogen

The immunogen is a synthetic peptide directed towards the C terminal region of rat TAAR4 (NP_783173.1). Peptide sequence FLWLGYFNSTFNPIIYGMFYPWFRKALRMIVTGTIFRSDSSTSSLHPAHP

Clonality

Polyclonal

Host

Rabbit

Theoretical MW

38 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for TAAR4 Antibody - BSA Free

Western Blot: TAAR4 Antibody [NBP3-10140]

Western Blot: TAAR4 Antibody [NBP3-10140]

Western Blot: TAAR4 Antibody [NBP3-10140] - Western blot analysis of TAAR4 in Rat Kidney lysates. Antibody dilution at 1ug/ml
TAAR4 Antibody - BSA Free

Western Blot: TAAR4 Antibody - BSA Free [NBP3-10140] -

TAAR1 mediates the tyramine-induced reduction in IA. a Western blot analysis of TAAR1 and TAAR4 protein expression in mouse TGs. beta -actin was used as an internal loading control. The immunoblots are representative of the results of at least three independent experiments. b Colocalization of TAAR1 (red) with NeuN, GS, IB4, CGRP or NF200 (green) in the TG (arrows). Scale bar = 50 μm. c and d The time course curve (c) and the bar chart (d) showing that pretreatment of TG neurons with EPPTB (3 uM) prevented the 0.1 uM tyramine-induced IA decrease (n = 10 cells). EPPTB at 3 uM alone did not affect IA (n = 7 cells). Insets in panel c indicate the representative traces. The letters indicate the points used for sample traces. **p < 0.01 vs. tyramine, unpaired t test. e Summary of results indicating quantified IA current density under control conditions, during exposure to RO5263397, and during washout (n = 9 cells). *p < 0.05 vs. control, paired t test. f Western blot analysis of TAAR1 protein expression in the NC-siRNA or TAAR1 siRNA-treated (TAAR1-siRNA) groups. The immunoblots are representative of the results of at least three independent experiments. **p < 0.01 vs. NC-siRNA, unpaired t test. g Representative traces (left panel) and bar chart (right panel) showing that treatment with TAAR1-siRNA abrogated the 0.1 uM tyramine-induced IA response (n = 12 cells). *p < 0.05 vs. control + NC-siRNA, unpaired t test Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37158881), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for TAAR4 Antibody - BSA Free

Application
Recommended Usage

Western Blot

1.0 ug/ml

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 2% Sucrose

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TAAR4

FUNCTION: Orphan receptor. Could be a receptor for trace amines. Of the biogenic trace amine tested, activated only by beta-phenylethylamine (beta-PEA) and tryptamine; however, the response is of low potency. Trace amines are biogenic amines present in very low levels in mammalian tissues. Although some trace amines have clearly defined roles as neurotransmitters in invertebrates, the extent to which they function as true neurotransmitters in vertebrates has remained speculative. Trace amines are likely to be involved in a variety of physiological functions that have yet to be fully understood. This receptor seems to be mediated by the G(s)-class of G-proteins which activate adenylate cyclase. SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.

Alternate Names

Gm226, Ta2, TAAR 4

Gene Symbol

Taar4

Additional TAAR4 Products

Product Documents for TAAR4 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for TAAR4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for TAAR4 Antibody - BSA Free

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