![Western Blot: TAMRA Antibody (5G5) [NBP2-22472]](https://resources.rndsystems.com/images/products/Rhodamine-Antibody-5G5-Western-Blot-NBP2-22472-img0001.jpg "Western Blot: TAMRA Antibody (5G5) [NBP2-22472]")
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Key Product Details
Species Reactivity
Non-species specific
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 5G5
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Product Specifications
Immunogen
TAMRA molecule
Reactivity Notes
This antibody reacts with Chemical.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for TAMRA Antibody (5G5)
Western Blot: TAMRA Antibody (5G5) [NBP2-22472]
Western Blot: Rhodamine Antibody (5G5) [NBP2-22472] - Analysis of various amounts of purified TAMRA-BSA protein.![Immunoprecipitation: TAMRA Antibody (5G5) [NBP2-22472]](https://resources.rndsystems.com/images/products/Rhodamine-Antibody-5G5-Immunoprecipitation-NBP2-22472-img0003.jpg "Immunoprecipitation: TAMRA Antibody (5G5) [NBP2-22472]")
Immunoprecipitation: TAMRA Antibody (5G5) [NBP2-22472]
Immunoprecipitation: Rhodamine Antibody (5G5) [NBP2-22472] - Analysis was performed on purified TAMRA-BSA. The antigen: antibody complex was formed by binding 5ug protein with 10ug of mouse monoclonal antibody recognizing TAMRA or Mouse IgG, whole molecule overnight on a rocking platform at 4C. The immune-complex was then captured on 50ul Protein A/G Plus Agarose. Captured immune-complexes were then washed extensively and proteins eluted with 5X Reducing Sample Loading Dye. Samples were then resolved on a 4-20% Tris-HCl polyacrylamide gel. Proteins were imaged using Typhoon 9410 Imager at 532nM, Normal Sensitivity.Applications for TAMRA Antibody (5G5)
Application
Recommended Usage
Immunoprecipitation
10 ug
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS with 1 mg/ml BSA
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: TAMRA
Alternate Names
BP-25606, F07-0040, TAMRA Azide, TAMRA Azide, isomer 5, tetramethylrhodamine azide
Additional TAMRA Products
Product Documents for TAMRA Antibody (5G5)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for TAMRA Antibody (5G5)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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