TAO2 [p Ser181] Antibody - Azide Free

Novus Biologicals | Catalog # NB300-233

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Avian (100%), Canine (100%), Chicken (100%), Finch (100%), Xenopus (100%), Zebrafish (100%). Backed by our 100% Guarantee.

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide Free
View all TAO2 Primary Antibodies »

Product Specifications

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser181 conjugated to KLH. Accession # Q9UL54

Reactivity Notes

Reactivity assumed based on 100% sequence homology: Finch.

Modification

p Ser181

Localization

Integral membrane protein. Found to be perinuclear and localized to vesicular compartment

Specificity

Specific for endogenous levels of the ~120 kDa TAO2 phosphorylated at Ser181. Immunolabeling is completely eliminated by treatment with lambda-phosphatase.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

120 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit TAO2 [p Ser181] Antibody - Azide Free (NB300-233) is a polyclonal antibody validated for use in WB. Anti-TAO2 Antibody: Cited in 5 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for TAO2 [p Ser181] Antibody - Azide Free

Western Blot: TAO2 [p Ser181] Antibody [NB300-233]

Western Blot: TAO2 [p Ser181] Antibody [NB300-233]

Western Blot: TAO2 [p Ser181] Antibody [NB300-233] - Western blot of rat hippocampal lysate showing specific labeling of the ~120 kDa TAO Kinase 2 (phospho-Ser181) band.
Western Blot: TAO2 [p Ser181] Antibody [NB300-233]

Western Blot: TAO2 [p Ser181] Antibody [NB300-233]

Western Blot: TAO2 [p Ser181] Antibody [NB300-233] - Western blot of rat cortex lysate showing specific immunolabeling of the ~120k TAO2 phosphorylated at Ser181 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubatede (1200 units for 30 min) before being exposed to the Ser181 TAO2 antibody. The immunolabeling is completely eliminated by treatment with lambda-phosphatase.

Applications for TAO2 [p Ser181] Antibody - Azide Free

Application
Recommended Usage

Western Blot

1:1000
Application Notes
Western The investigator should determine the optimal working dilution for a specific application.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol

Format

Azide Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TAO2

In vitro, TAO (thousand and one amino acid) protein kinase 2 (TAO2) activates MAP/ERK kinases (MEKs) 3, 4, and 6 toward their substrates p38 MAP kinase JNK/SAPK (Chen et al., 1999; Chen and Cobb, 2001). This and more recent work has led to the proposal that the TAO protein kinases play an essential role in signaling from physiological agonists to the stress-responsive p38 MAPKs (Chen et al., 2003). Autophosphorylation of TAO may play a role in the mechanism of TAO activation. The MEK binding domain of TAO is autophosphorylated on both serine and threonine residues and Ser181 is located within this domain.

Long Name

Serine/Threonine-protein Kinase TAO2

Alternate Names

KFC-C, MAP3K17, PSK1, TAO1, TAOK2

Entrez Gene IDs

64666 (Rat)

Gene Symbol

TAOK2

UniProt

Q9UL54 (Human)

Additional TAO2 Products

Product Documents for TAO2 [p Ser181] Antibody - Azide Free

Certificate of Analysis

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Product Specific Notices for TAO2 [p Ser181] Antibody - Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Intracellular Kinases

Citations for TAO2 [p Ser181] Antibody - Azide Free

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