TAT Antibody (OTI3G6)

Novus Biologicals | Catalog # NBP3-44843

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI3G6
View all TAT Primary Antibodies »

Product Specifications

Immunogen

Full length human recombiant protein of human TAT (NP_000344) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for TAT Antibody (OTI3G6)

TAT Antibody (OTI3G6) Western Blot;TAT Antibody (OTI3G6)[NBP3-44843]-

Western Blot;TAT Antibody (OTI3G6)[NBP3-44843]-

HEK293T cells were transfected with the pCMV6-ENTRY control ( Left lane) or pCMV6-ENTRY TAT ( Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TAT(1:2000). Positive lysates

Applications for TAT Antibody (OTI3G6)

Application
Recommended Usage

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

>95%

Reconstitution

For reconstitution, we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized powder ( 1X PBS, pH 7.3,)

Preservative

8% Trehalose

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 degrees C. Avoid freeze/thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TAT

The transcriptional transactivator (Tat) is a key regulatory protein of HIV. It is expressed early after the virus integrates into the cell, and stimulates the elongation of RNA polymerase II. It binds onto a sequence known as the TAR, or transactivator response element, located at the end of the HIV genetic chain. There, the tat protein helps assemble new copies of HIV. The tat protein-TAR complex speeds up the rate of viral reproduction by about a thousand times. If it is not present, the transcription process frequently stops short, and few functional HIV particles are produced. Tat is an important potential target for antiretrovirals and vaccine development. Transcriptional regulator that acts by binding to the trans-activating responsive sequence (TAR) RNA element and activates transcription initiation and/or elongation from the LTR promoter.

Alternate Names

EC 2.6.1.5, L-tyrosine:2-oxoglutarate aminotransferase, tyrosine aminotransferase, tyrosine aminotransferase, cytosolic

Gene Symbol

TAT

Additional TAT Products

Product Documents for TAT Antibody (OTI3G6)

Certificate of Analysis

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Product Specific Notices for TAT Antibody (OTI3G6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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