Human TBK1 - Ready-To-Use ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP3-40173

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Tissue homogenates, cell lysates and other biological fluids (100 uL)

Sensitivity

0.112 ng/mL (example only; lot dependent)

Assay Range

0.312 - 20 ng/mL (example only; lot dependent)
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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Human

Description

The Ready-To-Use ELISA kit offers pre-diluted detection reagents and a shorter experimental time.
Assay Length: 3 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV 10 (example only; lot dependent)

Inter-Assay Precision (Precision between assays) %CV 12 (example only; lot dependent)

Scientific Data Images for Human TBK1 - Ready-To-Use ELISA Kit (Colorimetric)

Human TBK1 - Ready-To-Use ELISA Kit (Colorimetric)

ELISA: Human TBK1 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-40173]

ELISA: Human TBK1 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-40173] - Standard Curve Reference

Kit Contents for Human TBK1 - Ready-To-Use ELISA Kit (Colorimetric)

  • Detection Solution A
  • Detection Solution B
  • Instruction manual
  • Plate sealer for 96 wells
  • Pre-coated 96T strip plate
  • Standard
  • Standard Diluent
  • Stop Solution
  • TMB Substrate
  • Wash Buffer (30 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: TBK1

NFkappaB-activating kinase (NAK) also known as TANK binding kinase-1 (TBK1) or tumor necrosis factor receptor associated factor 2-associated kinase (T2K) is a serine/threonine protein that belongs to the IkB kinase (IKK) family (1). Similar to IKK alpha and beta (2), NAK induces IkB degradation and NF-kB activity. NAK is activated by phorbol ester tumour promoters and growth factors, whereas catalytically inactive NAK specifically inhibits activation of NF-kB by protein kinase C-epsilon (PKCepsilon). NAK specifically phosphorylates IkB alpha on Serine 36 and NFkB subunit RelA (p65) on Serine 536 (3). NAK is also known to interact with TANK, TRAF-2 and TRIF (4).

Long Name

TANK Binding Kinase 1

Alternate Names

FTDALS4, NAK, NFKB-Activating Kinase

Gene Symbol

TBK1

Additional TBK1 Products

Product Documents for Human TBK1 - Ready-To-Use ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Human TBK1 - Ready-To-Use ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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FAQs for Human TBK1 - Ready-To-Use ELISA Kit (Colorimetric)

Showing  1 - 5 of 6 FAQs Showing All
  • Q: Are there any publications where this TBK1 antibody was used in western blot for mouse?

    A: This TBK1 antibody was cited in a study looking at the relation between loss of TBK1 activity and increased susceptibility to LPS induced lethality. PMID 20651301

  • Q: Cant this TBK1 antibody be conjugated to PE for FLOW?

    A: Although it has not been validated for flow we do offer this TBK1 antibody as a PE conjugate. If you would like to test in FLOW you may want to take advantage of our innovator's reward program.

  • Q: I'm using NAX (TBK1 antibody) why is my band different from the predicted molecular weight?

    A: While this TBK1 antibody is typically observed around 83 kDA variations including PTMs, relative charges and other experimental factors can affect where the band is observed.

  • Q: Is there a smaller size of your TBK1 antibody?

    A: This TBK1 antibody is offered in a smaller 0.025 mg size.

  • Q: We are looking to use your TBK1 antibody in simple western but need a good loading control also validated in simple western, which would you recommend?

    A: Our alpha tubulin loading control antibody (NB100-690) would be a good choice to use with the TBK1 antibody; it detects around 55 kDa and is validated in simple western.

  • Q: What is the exact sequence this TBK1 antibody recognizes?

    A: The exact epitope of this TBK1 antibody is unknown because we do not perform epitope mapping. This TBK1 antibody was made against a synthetic peptide corresponding to aa 563-577 of human TBK1.

  • Q: Are there any publications where this TBK1 antibody was used in western blot for mouse?

    A: This TBK1 antibody was cited in a study looking at the relation between loss of TBK1 activity and increased susceptibility to LPS induced lethality. PMID 20651301

  • Q: Cant this TBK1 antibody be conjugated to PE for FLOW?

    A: Although it has not been validated for flow we do offer this TBK1 antibody as a PE conjugate. If you would like to test in FLOW you may want to take advantage of our innovator's reward program.

  • Q: I'm using NAX (TBK1 antibody) why is my band different from the predicted molecular weight?

    A: While this TBK1 antibody is typically observed around 83 kDA variations including PTMs, relative charges and other experimental factors can affect where the band is observed.

  • Q: Is there a smaller size of your TBK1 antibody?

    A: This TBK1 antibody is offered in a smaller 0.025 mg size.

  • Q: We are looking to use your TBK1 antibody in simple western but need a good loading control also validated in simple western, which would you recommend?

    A: Our alpha tubulin loading control antibody (NB100-690) would be a good choice to use with the TBK1 antibody; it detects around 55 kDa and is validated in simple western.

  • Q: What is the exact sequence this TBK1 antibody recognizes?

    A: The exact epitope of this TBK1 antibody is unknown because we do not perform epitope mapping. This TBK1 antibody was made against a synthetic peptide corresponding to aa 563-577 of human TBK1.

  • Q: Are there any publications where this TBK1 antibody was used in western blot for mouse?

    A: This TBK1 antibody was cited in a study looking at the relation between loss of TBK1 activity and increased susceptibility to LPS induced lethality. PMID 20651301

  • Q: Cant this TBK1 antibody be conjugated to PE for FLOW?

    A: Although it has not been validated for flow we do offer this TBK1 antibody as a PE conjugate. If you would like to test in FLOW you may want to take advantage of our innovator's reward program.

  • Q: I'm using NAX (TBK1 antibody) why is my band different from the predicted molecular weight?

    A: While this TBK1 antibody is typically observed around 83 kDA variations including PTMs, relative charges and other experimental factors can affect where the band is observed.

  • Q: Is there a smaller size of your TBK1 antibody?

    A: This TBK1 antibody is offered in a smaller 0.025 mg size.

  • Q: We are looking to use your TBK1 antibody in simple western but need a good loading control also validated in simple western, which would you recommend?

    A: Our alpha tubulin loading control antibody (NB100-690) would be a good choice to use with the TBK1 antibody; it detects around 55 kDa and is validated in simple western.

  • Q: What is the exact sequence this TBK1 antibody recognizes?

    A: The exact epitope of this TBK1 antibody is unknown because we do not perform epitope mapping. This TBK1 antibody was made against a synthetic peptide corresponding to aa 563-577 of human TBK1.

  • Q: Are there any publications where this TBK1 antibody was used in western blot for mouse?

    A: This TBK1 antibody was cited in a study looking at the relation between loss of TBK1 activity and increased susceptibility to LPS induced lethality. PMID 20651301

  • Q: Cant this TBK1 antibody be conjugated to PE for FLOW?

    A: Although it has not been validated for flow we do offer this TBK1 antibody as a PE conjugate. If you would like to test in FLOW you may want to take advantage of our innovator's reward program.

  • Q: I'm using NAX (TBK1 antibody) why is my band different from the predicted molecular weight?

    A: While this TBK1 antibody is typically observed around 83 kDA variations including PTMs, relative charges and other experimental factors can affect where the band is observed.

  • Q: Is there a smaller size of your TBK1 antibody?

    A: This TBK1 antibody is offered in a smaller 0.025 mg size.

  • Q: We are looking to use your TBK1 antibody in simple western but need a good loading control also validated in simple western, which would you recommend?

    A: Our alpha tubulin loading control antibody (NB100-690) would be a good choice to use with the TBK1 antibody; it detects around 55 kDa and is validated in simple western.

  • Q: What is the exact sequence this TBK1 antibody recognizes?

    A: The exact epitope of this TBK1 antibody is unknown because we do not perform epitope mapping. This TBK1 antibody was made against a synthetic peptide corresponding to aa 563-577 of human TBK1.

  • Q: Are there any publications where this TBK1 antibody was used in western blot for mouse?

    A: This TBK1 antibody was cited in a study looking at the relation between loss of TBK1 activity and increased susceptibility to LPS induced lethality. PMID 20651301

  • Q: Cant this TBK1 antibody be conjugated to PE for FLOW?

    A: Although it has not been validated for flow we do offer this TBK1 antibody as a PE conjugate. If you would like to test in FLOW you may want to take advantage of our innovator's reward program.

  • Q: I'm using NAX (TBK1 antibody) why is my band different from the predicted molecular weight?

    A: While this TBK1 antibody is typically observed around 83 kDA variations including PTMs, relative charges and other experimental factors can affect where the band is observed.

  • Q: Is there a smaller size of your TBK1 antibody?

    A: This TBK1 antibody is offered in a smaller 0.025 mg size.

  • Q: We are looking to use your TBK1 antibody in simple western but need a good loading control also validated in simple western, which would you recommend?

    A: Our alpha tubulin loading control antibody (NB100-690) would be a good choice to use with the TBK1 antibody; it detects around 55 kDa and is validated in simple western.

  • Q: What is the exact sequence this TBK1 antibody recognizes?

    A: The exact epitope of this TBK1 antibody is unknown because we do not perform epitope mapping. This TBK1 antibody was made against a synthetic peptide corresponding to aa 563-577 of human TBK1.

  • Q: Are there any publications where this TBK1 antibody was used in western blot for mouse?

    A: This TBK1 antibody was cited in a study looking at the relation between loss of TBK1 activity and increased susceptibility to LPS induced lethality. PMID 20651301

  • Q: Cant this TBK1 antibody be conjugated to PE for FLOW?

    A: Although it has not been validated for flow we do offer this TBK1 antibody as a PE conjugate. If you would like to test in FLOW you may want to take advantage of our innovator's reward program.

  • Q: I'm using NAX (TBK1 antibody) why is my band different from the predicted molecular weight?

    A: While this TBK1 antibody is typically observed around 83 kDA variations including PTMs, relative charges and other experimental factors can affect where the band is observed.

  • Q: Is there a smaller size of your TBK1 antibody?

    A: This TBK1 antibody is offered in a smaller 0.025 mg size.

  • Q: We are looking to use your TBK1 antibody in simple western but need a good loading control also validated in simple western, which would you recommend?

    A: Our alpha tubulin loading control antibody (NB100-690) would be a good choice to use with the TBK1 antibody; it detects around 55 kDa and is validated in simple western.

  • Q: What is the exact sequence this TBK1 antibody recognizes?

    A: The exact epitope of this TBK1 antibody is unknown because we do not perform epitope mapping. This TBK1 antibody was made against a synthetic peptide corresponding to aa 563-577 of human TBK1.

Showing  1 - 5 of 6 FAQs Showing All
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