TBK1 Overexpression Lysate
Novus Biologicals | Catalog # NBP2-10346
![Western Blot: TBK1 Overexpression Lysate [NBP2-10346]](https://resources.rndsystems.com/images/products/NAK-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-10346-img0001.jpg "Western Blot: TBK1 Overexpression Lysate [NBP2-10346]")
Key Product Details
Species
Applications
Product Summary for TBK1 Overexpression Lysate
TBK1 Transient Overexpression Lysate
Expression Host: HEK293T
Plasmid: RC205238
Accession#: NM_013254
Protein Tag: C-MYC/DDK
You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
Product Specifications
Application Notes
This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.
Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Theoretical MW
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Type
Scientific Data Images for TBK1 Overexpression Lysate
Western Blot: TBK1 Overexpression Lysate [NBP2-10346]
Western Blot: NAK Overexpression Lysate (Adult Normal) [NBP2-10346] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NAK.Formulation, Preparation, and Storage
Formulation
Concentration
Shipping
Storage
Background: TBK1
Long Name
Alternate Names
Gene Symbol
Additional TBK1 Products
Product Documents for TBK1 Overexpression Lysate
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for TBK1 Overexpression Lysate
HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for TBK1 Overexpression Lysate
-
Q: Are there any publications where this TBK1 antibody was used in western blot for mouse?
A: This TBK1 antibody was cited in a study looking at the relation between loss of TBK1 activity and increased susceptibility to LPS induced lethality. PMID 20651301
-
Q: Cant this TBK1 antibody be conjugated to PE for FLOW?
A: Although it has not been validated for flow we do offer this TBK1 antibody as a PE conjugate. If you would like to test in FLOW you may want to take advantage of our innovator's reward program.
-
Q: I'm using NAX (TBK1 antibody) why is my band different from the predicted molecular weight?
A: While this TBK1 antibody is typically observed around 83 kDA variations including PTMs, relative charges and other experimental factors can affect where the band is observed.
-
Q: Is there a smaller size of your TBK1 antibody?
A: This TBK1 antibody is offered in a smaller 0.025 mg size.
-
Q: We are looking to use your TBK1 antibody in simple western but need a good loading control also validated in simple western, which would you recommend?
A: Our alpha tubulin loading control antibody (NB100-690) would be a good choice to use with the TBK1 antibody; it detects around 55 kDa and is validated in simple western.
-
Q: What is the exact sequence this TBK1 antibody recognizes?
A: The exact epitope of this TBK1 antibody is unknown because we do not perform epitope mapping. This TBK1 antibody was made against a synthetic peptide corresponding to aa 563-577 of human TBK1.
-
Q: Are there any publications where this TBK1 antibody was used in western blot for mouse?
A: This TBK1 antibody was cited in a study looking at the relation between loss of TBK1 activity and increased susceptibility to LPS induced lethality. PMID 20651301
-
Q: Cant this TBK1 antibody be conjugated to PE for FLOW?
A: Although it has not been validated for flow we do offer this TBK1 antibody as a PE conjugate. If you would like to test in FLOW you may want to take advantage of our innovator's reward program.
-
Q: I'm using NAX (TBK1 antibody) why is my band different from the predicted molecular weight?
A: While this TBK1 antibody is typically observed around 83 kDA variations including PTMs, relative charges and other experimental factors can affect where the band is observed.
-
Q: Is there a smaller size of your TBK1 antibody?
A: This TBK1 antibody is offered in a smaller 0.025 mg size.
-
Q: We are looking to use your TBK1 antibody in simple western but need a good loading control also validated in simple western, which would you recommend?
A: Our alpha tubulin loading control antibody (NB100-690) would be a good choice to use with the TBK1 antibody; it detects around 55 kDa and is validated in simple western.
-
Q: What is the exact sequence this TBK1 antibody recognizes?
A: The exact epitope of this TBK1 antibody is unknown because we do not perform epitope mapping. This TBK1 antibody was made against a synthetic peptide corresponding to aa 563-577 of human TBK1.
-
Q: Are there any publications where this TBK1 antibody was used in western blot for mouse?
A: This TBK1 antibody was cited in a study looking at the relation between loss of TBK1 activity and increased susceptibility to LPS induced lethality. PMID 20651301
-
Q: Cant this TBK1 antibody be conjugated to PE for FLOW?
A: Although it has not been validated for flow we do offer this TBK1 antibody as a PE conjugate. If you would like to test in FLOW you may want to take advantage of our innovator's reward program.
-
Q: I'm using NAX (TBK1 antibody) why is my band different from the predicted molecular weight?
A: While this TBK1 antibody is typically observed around 83 kDA variations including PTMs, relative charges and other experimental factors can affect where the band is observed.
-
Q: Is there a smaller size of your TBK1 antibody?
A: This TBK1 antibody is offered in a smaller 0.025 mg size.
-
Q: We are looking to use your TBK1 antibody in simple western but need a good loading control also validated in simple western, which would you recommend?
A: Our alpha tubulin loading control antibody (NB100-690) would be a good choice to use with the TBK1 antibody; it detects around 55 kDa and is validated in simple western.
-
Q: What is the exact sequence this TBK1 antibody recognizes?
A: The exact epitope of this TBK1 antibody is unknown because we do not perform epitope mapping. This TBK1 antibody was made against a synthetic peptide corresponding to aa 563-577 of human TBK1.
-
Q: Are there any publications where this TBK1 antibody was used in western blot for mouse?
A: This TBK1 antibody was cited in a study looking at the relation between loss of TBK1 activity and increased susceptibility to LPS induced lethality. PMID 20651301
-
Q: Cant this TBK1 antibody be conjugated to PE for FLOW?
A: Although it has not been validated for flow we do offer this TBK1 antibody as a PE conjugate. If you would like to test in FLOW you may want to take advantage of our innovator's reward program.
-
Q: I'm using NAX (TBK1 antibody) why is my band different from the predicted molecular weight?
A: While this TBK1 antibody is typically observed around 83 kDA variations including PTMs, relative charges and other experimental factors can affect where the band is observed.
-
Q: Is there a smaller size of your TBK1 antibody?
A: This TBK1 antibody is offered in a smaller 0.025 mg size.
-
Q: We are looking to use your TBK1 antibody in simple western but need a good loading control also validated in simple western, which would you recommend?
A: Our alpha tubulin loading control antibody (NB100-690) would be a good choice to use with the TBK1 antibody; it detects around 55 kDa and is validated in simple western.
-
Q: What is the exact sequence this TBK1 antibody recognizes?
A: The exact epitope of this TBK1 antibody is unknown because we do not perform epitope mapping. This TBK1 antibody was made against a synthetic peptide corresponding to aa 563-577 of human TBK1.
-
Q: Are there any publications where this TBK1 antibody was used in western blot for mouse?
A: This TBK1 antibody was cited in a study looking at the relation between loss of TBK1 activity and increased susceptibility to LPS induced lethality. PMID 20651301
-
Q: Cant this TBK1 antibody be conjugated to PE for FLOW?
A: Although it has not been validated for flow we do offer this TBK1 antibody as a PE conjugate. If you would like to test in FLOW you may want to take advantage of our innovator's reward program.
-
Q: I'm using NAX (TBK1 antibody) why is my band different from the predicted molecular weight?
A: While this TBK1 antibody is typically observed around 83 kDA variations including PTMs, relative charges and other experimental factors can affect where the band is observed.
-
Q: Is there a smaller size of your TBK1 antibody?
A: This TBK1 antibody is offered in a smaller 0.025 mg size.
-
Q: We are looking to use your TBK1 antibody in simple western but need a good loading control also validated in simple western, which would you recommend?
A: Our alpha tubulin loading control antibody (NB100-690) would be a good choice to use with the TBK1 antibody; it detects around 55 kDa and is validated in simple western.
-
Q: What is the exact sequence this TBK1 antibody recognizes?
A: The exact epitope of this TBK1 antibody is unknown because we do not perform epitope mapping. This TBK1 antibody was made against a synthetic peptide corresponding to aa 563-577 of human TBK1.
-
Q: Are there any publications where this TBK1 antibody was used in western blot for mouse?
A: This TBK1 antibody was cited in a study looking at the relation between loss of TBK1 activity and increased susceptibility to LPS induced lethality. PMID 20651301
-
Q: Cant this TBK1 antibody be conjugated to PE for FLOW?
A: Although it has not been validated for flow we do offer this TBK1 antibody as a PE conjugate. If you would like to test in FLOW you may want to take advantage of our innovator's reward program.
-
Q: I'm using NAX (TBK1 antibody) why is my band different from the predicted molecular weight?
A: While this TBK1 antibody is typically observed around 83 kDA variations including PTMs, relative charges and other experimental factors can affect where the band is observed.
-
Q: Is there a smaller size of your TBK1 antibody?
A: This TBK1 antibody is offered in a smaller 0.025 mg size.
-
Q: We are looking to use your TBK1 antibody in simple western but need a good loading control also validated in simple western, which would you recommend?
A: Our alpha tubulin loading control antibody (NB100-690) would be a good choice to use with the TBK1 antibody; it detects around 55 kDa and is validated in simple western.
-
Q: What is the exact sequence this TBK1 antibody recognizes?
A: The exact epitope of this TBK1 antibody is unknown because we do not perform epitope mapping. This TBK1 antibody was made against a synthetic peptide corresponding to aa 563-577 of human TBK1.