TcBuster-M Transposase Alexa Fluor™ Plus 405‑conjugated Antibody

R&D Systems | Catalog # FAB11511AFP405

R&D Systems

Key Product Details

Species Reactivity

Multi-Species

Applications

Western Blot

Label

Alexa Fluor Plus 405 (Excitation = 404 nm, Emission = 455 nm)

Antibody Source

Recombinant Monoclonal Mouse IgG2B Clone # 1077035
View all TcBuster Transposase Primary Antibodies »

Product Specifications

Specificity

Detects TcBuster-M Transposase in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for TcBuster-M Transposase Alexa Fluor™ Plus 405‑conjugated Antibody

Application
Recommended Usage

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: TcBuster Transposase

TcBuster is a non-viral gene delivery system that enables stable gene transfer in most cell types. The TcBuster system belongs to the hAT-family of DNA transposons and is derived from the red flour beetle Tribolium castaneum (1). It consists of the TcBuster transposase mRNA and DNA transposon encoding multicistronic cargos for delivery. This antibody binds to the TcBuster-M transposase enzyme, a hyperactive version of TcBuster and carriers out the cut-and-paste reactions for efficient delivery of DNA cargos (2). TcBuster is used in a wide variety of genome engineering applications for both research and commercial purposes. These include, but are not limited to, immune cell therapies (T, NK cells), stem cell therapies for regenerative medicine (iPSCs), and bioproduction of biologics, such as monoclonal antibodies. The TcBuster system is available for purchase from Bio-Techne. For more information, please visit https://www.bio-techne.com/services/gene-engineering-services-tcbuster">https://www.bio-techne.com/services/gene-engineering-services-tcbuster.

References

  1. Woodard, L.E. et al. (2012) PLOS One DOI: 10.1371/journal.pone.0042666.
  2. Skeate, J.G. et al. (2024) Molecular Therapy DOI: 10.1016/j.ymthe.2024.04.024.

Long Name

Tribolium castaneumTcBuster Transposase Peptide

Alternate Names

GLEAN_06077, TcasGA2_TC006077, Transposase

Additional TcBuster Transposase Products

Product Documents for TcBuster-M Transposase Alexa Fluor™ Plus 405‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for TcBuster-M Transposase Alexa Fluor™ Plus 405‑conjugated Antibody


This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only

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