TcBuster Transposase Antibody (1077035)

R&D Systems | Catalog # MAB11511

R&D Systems

Key Product Details

Species Reactivity

Multi-Species

Applications

Western Blot, Simple Western

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 1077035
View all TcBuster Transposase Primary Antibodies »

Product Specifications

Immunogen

Synthetic Peptide

Specificity

Detects TcBuster-M Transposase in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for TcBuster Transposase Antibody (1077035)

Detection of TcBuster-M Transposase by Western Blot.

Western Blot shows lysates of HEK293T human embryonic kidney cell line mock electroporated or electroporated with 1 µg TcBuster-M Transposase after 1 day post-electroporation. PVDF membrane was probed with 1 µg/ml of Mouse Anti-TcBuster-M Transposase Monoclonal Antibody (Catalog # MAB11511) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for TcBuster-M Transposase at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Detection of TcBuster-M Transposase by Simple WesternTM.

Simple Western shows lysates HEK293T human embryonic kidney cell line mock electroporated or electroporated with 1µg TcBuster-M Transposase after 1 day post-electroporation, loaded at 0.5 mg/ml. A specific band was detected for TcBuster-M Transposase at approximately 71 kDa (as indicated) using 20 µg/mL of Mouse Anti-TcBuster-M Transposase Monoclonal Antibody (Catalog # MAB11511). This experiment was conducted under reducing conditions and using the 12‑230 kDa separation system.

Applications for TcBuster Transposase Antibody (1077035)

Application
Recommended Usage

Simple Western

20 µg/mL
Sample: HEK293T human embryonic kidney cell line mock electroporated or electroporated with 1 µg TcBuster-M Transposase after 1 day post-electroporation

Western Blot

1 µg/mL
Sample: HEK293T human embryonic kidney cell line mock electroporated or electroporated with 1 µg TcBuster-M Transposase after 1 day post-electroporation

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TcBuster Transposase

TcBuster is a non-viral gene delivery system that enables stable gene transfer in most cell types. The TcBuster system belongs to the hAT-family of DNA transposons and is derived from the red flour beetle Tribolium castaneum (1). It consists of the TcBuster transposase mRNA and DNA transposon encoding multicistronic cargos for delivery. This antibody binds to the TcBuster-M transposase enzyme, a hyperactive version of TcBuster and carriers out the cut-and-paste reactions for efficient delivery of DNA cargos (2). TcBuster is used in a wide variety of genome engineering applications for both research and commercial purposes. These include, but are not limited to, immune cell therapies (T, NK cells), stem cell therapies for regenerative medicine (iPSCs), and bioproduction of biologics, such as monoclonal antibodies. The TcBuster system is available for purchase from Bio-Techne. For more information, please visit https://www.bio-techne.com/services/gene-engineering-services-tcbuster">https://www.bio-techne.com/services/gene-engineering-services-tcbuster.

References

  1. Woodard, L.E. et al. (2012) PLOS One DOI: 10.1371/journal.pone.0042666.
  2. Skeate, J.G. et al. (2024) Molecular Therapy DOI: 10.1016/j.ymthe.2024.04.024.

Long Name

Tribolium castaneumTcBuster Transposase Peptide

Alternate Names

GLEAN_06077, TcasGA2_TC006077, Transposase

Additional TcBuster Transposase Products

Product Documents for TcBuster Transposase Antibody (1077035)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for TcBuster Transposase Antibody (1077035)

For research use only

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