TCP1 alpha Antibody (2B2-D6) - Azide and BSA Free

Novus Biologicals | Catalog # H00006950-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2B2-D6

Format

Azide and BSA Free
View all TCP1 alpha Primary Antibodies »

Product Specifications

Immunogen

TCP1 (AAH00665, 1 a.a. ~ 556 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MEGPLSVFGDRSTGETIRSQNVMAAASIANIVKSSFGPVGLDKMLVDDIGDVTITNDGATILKLLEVEHPAAKVLCELADLQDKEVGDGTTSVVIIAAELLKNADELVKQKIHPTSVISGYRLACKEAVRYINENLIVNTDELGRDCLINAAKTSMSSKIIGINGDFFANMVVDAVLAIKYTDIRGQPRYPVNSVNILKAHGRSQMESMLISGYALNCVVGSQGMPKRIVNAKIACLDFSLQKTKMKLGVQVVITDPEKLDQIRQRESDITKERIQKILATGANVILTTGGIDDMCLKYFVEAGAMAVRRVLKRDLKRIAKASGATILSTLANLEGEETFEAAMLGQAEEVVQERICDDELILIKNTKARTSASIILRGANDFMCDEMERSLHDALCVVKRVLESKSVVPGGGAVEAALSIYLENYATSMGSREQLAIAEFARSLLVIPNTLAVNAAQDSTDLVAKLRAFHNEAQVNPERKNLKWIGLDLSNGKPRDNKQAGVFEPTIVKVKSLKFATEAAITILRIDDLIKLHPESKDDKHGSYEDAVHSGALND

Specificity

TCP1 - t-complex 1

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for TCP1 alpha Antibody (2B2-D6) - Azide and BSA Free

Western Blot: TCP1 alpha Antibody (2B2-D6) [H00006950-M01]

Western Blot: TCP1 alpha Antibody (2B2-D6) [H00006950-M01]

Western Blot: TCP1 alpha Antibody (2B2-D6) [H00006950-M01] - TCP1 monoclonal antibody (M01), clone 2B2-D6 Analysis of TCP1 expression in Hela.
Immunocytochemistry/ Immunofluorescence: TCP1 alpha Antibody (2B2-D6) [H00006950-M01]

Immunocytochemistry/ Immunofluorescence: TCP1 alpha Antibody (2B2-D6) [H00006950-M01]

Immunocytochemistry/Immunofluorescence: TCP1 alpha Antibody (2B2-D6) [H00006950-M01] - Analysis of monoclonal antibody to TCP1 on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: TCP1 alpha Antibody (2B2-D6) [H00006950-M01]

Immunohistochemistry-Paraffin: TCP1 alpha Antibody (2B2-D6) [H00006950-M01]

Immunohistochemistry-Paraffin: TCP1 alpha Antibody (2B2-D6) [H00006950-M01] - Analysis of monoclonal antibody to TCP1 on formalin-fixed paraffin-embedded human tonsil. Antibody concentration 3 ug/ml.
ELISA: TCP1 alpha Antibody (2B2-D6) [H00006950-M01]

ELISA: TCP1 alpha Antibody (2B2-D6) [H00006950-M01]

ELISA: TCP1 alpha Antibody (2B2-D6) [H00006950-M01] - Detection limit for recombinant GST tagged TCP1 is approximately 0.1ng/ml as a capture antibody.

Applications for TCP1 alpha Antibody (2B2-D6) - Azide and BSA Free

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, IHC-P and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: TCP1 alpha

This gene encodes a molecular chaperone that is member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants of this gene, encoding different isoforms, have been characterized.

Alternate Names

CCT1T-complex protein 1 subunit alpha, CCTA, CCT-alpha, D6S230E, tailless complex polypeptide 1, t-complex 1, T-complex protein 1, alpha subunit, TCP-1-alpha

Entrez Gene IDs

6950 (Human)

Gene Symbol

TCP1

OMIM

186980 (Human)

UniProt

AAH00665 (Human)

Additional TCP1 alpha Products

Product Documents for TCP1 alpha Antibody (2B2-D6) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for TCP1 alpha Antibody (2B2-D6) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for TCP1 alpha Antibody (2B2-D6) - Azide and BSA Free

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Protocols

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FAQs

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