TCP1 alpha Antibody

Name: TCP1 alpha Antibody
Brand: Novus Biologicals
SKU: NB100-1400
Price: 399 USD
Availability: InStock

Novus Biologicals | Catalog # NB100-1400

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Product Documents

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Peptide ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

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Product Specifications

Immunogen

Peptide with sequence C-SYEDAVHSGALND corresponding to C-Terminus according to NP_110379.2, NP_001008897.1.

Localization

Cytoplasmic

Specificity

This antibody is expected to recognize both reported isoforms.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for TCP1 alpha Antibody

Western Blot: TCP1 alpha Antibody [NB100-1400]

Western Blot: TCP1 alpha Antibody [NB100-1400] - Staining of Human Ovary (A) lysate and HEK293 (B) cell lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.

Immunocytochemistry/ Immunofluorescence: TCP1 alpha Antibody [NB100-1400]

Immunocytochemistry/Immunofluorescence: TCP1 alpha Antibody [NB100-1400] - Analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Immunohistochemistry-Paraffin: TCP1 alpha Antibody [NB100-1400]

Immunohistochemistry-Paraffin: TCP1 alpha Antibody [NB100-1400] - Staining of paraffin embedded Human Testis. Antibody at 10 ug/mL. Microwaved antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.

Flow Cytometry: TCP1 alpha Antibody [NB100-1400]

Flow Cytometry: TCP1 alpha Antibody [NB100-1400] - Analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

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Applications for TCP1 alpha Antibody

Application

Recommended Usage

Flow Cytometry

10 ug/mL

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Immunohistochemistry

1 - 2 ug/mL

Immunohistochemistry-Paraffin

1 - 2 ug/mL

Peptide ELISA

Detection limit 1:32000

Western Blot

0.3 - 1 ug/mL

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Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
Spillover Popups - Visualize the spectra of individual fluorochromes
Antigen Density Selector - Match fluorochrome brightness with antigen density

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Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TCP1 alpha

T-complex polypeptide-1 (TCP1) is a ~60 kDa protein constitutively expressed in almost all eukaryotic cells, and is upregulated during spermatogenesis. It is found in the cytosol as a subunit of a hetero-oligomeric chaperone that is known to be involved in the folding of actin and tubulin. The family of proteins termed chaperonins act to recognize and stabilize polypeptide intermediates during folding, assembly and disassembly, and share many characteristics with Heat Shock Protein 70 (HSP 70) including high abundance, induction by environmental stress, and ATPase activity. The chaperonin family includes the mitochondrial HSP60, Escherichia coli GroEL, the plastid Rubisco-subunit binding protein, and the archaebacterial protein TF55.The TCP 1 sequence shows nearly 40% identity to TF55, but only minimal similarity to HSP60 and GroEL.

Alternate Names

CCT1T-complex protein 1 subunit alpha, CCTA, CCT-alpha, D6S230E, tailless complex polypeptide 1, t-complex 1, T-complex protein 1, alpha subunit, TCP-1-alpha

Entrez Gene IDs

6950 (Human)

Gene Symbol

TCP1

UniProt

NP_110379. (Human)

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Product Documents for TCP1 alpha Antibody

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Certificate of Analysis

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Product Specific Notices for TCP1 alpha Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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