TCR V beta 5 Antibody (MEM-262) [Alexa Fluor™ Plus 647]

Novus Biologicals | Catalog # NB500-520AFP647

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Flow Cytometry, Immunoprecipitation, CyTOF-ready

Label

Alexa Fluor Plus 647 (Excitation = 658 nm, Emission = 675 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # MEM-262
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Product Specifications

Immunogen

Human thymoma cell line HPB-ALL.

Specificity

The antibody MEM-262 recognizes beta chains of the TCR expressed by HPB-ALL cell line [carrying V(beta5.3)] and a small subset of peripheral blood T cells. This subset is larger than that recognized by other V(beta5.3)-specific antibodies.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Description

This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.

For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.

Applications for TCR V beta 5 Antibody (MEM-262) [Alexa Fluor™ Plus 647]

Application
Recommended Usage

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Recommended applications are based on validated applications from the unconjugated base product NB500-520. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Spectra Viewer

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Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: TCR V beta 5

The V beta5 TCR is a variant of the TCR beta chain. It belongs to Ig superfamily and is expressed by a subset of peripheral blood T cells and some T cell clones. TCR V beta5 has been reported to recognize some staphylococcus bacterial superantigens. MEM-262 antibody reacts with a variant of the TCR beta chain which is expressed on HPB-ALL cells (known expressing TCR V beta5.3). But the subpopulation of peripheral blood T cells recognized by this antibody is reported larger than other anti-TCR V beta5.3 antibodies.

Alternate Names

T cell receptor beta locus, T-cell antigen receptor, beta polypeptide, T-cell receptor, beta cluster, T-cell receptor, beta cluster, TCRB, TRB

Gene Symbol

TRB

Additional TCR V beta 5 Products

Product Documents for TCR V beta 5 Antibody (MEM-262) [Alexa Fluor™ Plus 647]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for TCR V beta 5 Antibody (MEM-262) [Alexa Fluor™ Plus 647]



This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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