TDAG8/GPR65 Antibody - BSA Free

Novus Biologicals | Catalog # NLS1627

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Primate (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all TDAG8/GPR65 Primary Antibodies »

Product Specifications

Immunogen

Synthetic 19 amino acid peptide from 3rd cytoplasmic domain of human TDAG8/GPR65.

Epitope

3rd cytoplasmic domain

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Gorilla (100%), Gibbon (95%), Marmoset (95%), Rabbit (84%).

Localization

Cell Membrane

Specificity

Human GPR65. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Product can be stored undiluted at 4C for up to 1 month.

Scientific Data Images for TDAG8/GPR65 Antibody - BSA Free

Immunohistochemistry-Paraffin: TDAG8/GPR65 Antibody - BSA Free [NLS1627]

Immunohistochemistry-Paraffin: TDAG8/GPR65 Antibody - BSA Free [NLS1627]

Immunohistochemistry-Paraffin: TDAG8/GPR65 Antibody [NLS1627] - Human Lymph Node, Hodgkins Lymphoma tissue after heat-induced antigen retrieval.
Immunohistochemistry-Paraffin: TDAG8/GPR65 Antibody - BSA Free [NLS1627]

Immunohistochemistry-Paraffin: TDAG8/GPR65 Antibody - BSA Free [NLS1627]

Immunohistochemistry-Paraffin: TDAG8/GPR65 Antibody [NLS1627] - Analysis of anti-GPR65 / TDAG8 antibody with human lymph node, Hodgkins lymphoma.
Immunohistochemistry-Paraffin: TDAG8/GPR65 Antibody - BSA Free [NLS1627]

Immunohistochemistry-Paraffin: TDAG8/GPR65 Antibody - BSA Free [NLS1627]

Immunohistochemistry-Paraffin: TDAG8/GPR65 Antibody [NLS1627] - Human tonsil tissue after heat-induced antigen retrieval.

Applications for TDAG8/GPR65 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

9 - 23 ug/ml
Application Notes
.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.

Background: TDAG8/GPR65

GPR65 (TDAG8), a Lysophospholipid/Lysosphingolipid Receptor, is a molecular target for psychosine, a toxic lipid formed by the breakdown of galactosylceramide (cerebroside). A build-up of psychosine, caused by the lack of galactosylceramidase, results in the death of the myelin-synthesizing cells, oligodendrocytes, which is associated with Krabbe disease. GPR65 has been reported in human in peripheral blood leukocytes, spleen, lymph node, and thymus. ESTs have been isolated from human B-cell/lung/testis, brain/lung/testis, pancreas/spleen, lung/spleen, leukocyte, embryo, kidney, liver/spleen, thyroid, and vessel libraries.

Long Name

G Protein-coupled Receptor 65

Alternate Names

GPR65, Psychosine Receptor

Entrez Gene IDs

8477 (Human)

Gene Symbol

GPR65

UniProt

Q8IYL9 (Human)

Additional TDAG8/GPR65 Products

Product Documents for TDAG8/GPR65 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for TDAG8/GPR65 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for TDAG8/GPR65 Antibody - BSA Free

There are currently no reviews for this product. Be the first to review TDAG8/GPR65 Antibody - BSA Free and earn rewards!

Have you used TDAG8/GPR65 Antibody - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

View specific protocols for TDAG8/GPR65 Antibody - BSA Free (NLS1627):

Immunohistochemistry Protocol for GPR65 Antibody (NLS1627):
Immunohistochemistry

1. Prepare tissue with formalin fixation and by embedding it in paraffin wax.
2. Make 4 um sections and place on pre-cleaned and charged microscope slides.
3. Heat in a tissue-drying oven for 45 minutes @ 60 degrees Celcius.
4. Deparaffinize the tissues by wash drying the slides in 3 changes of xylene for 5 minutes each @ RT.
5. Rehydrate the tissues by washing the slides in 3 changes of 100% alcohol for 3 minutes each @ RT.
6. Wash the slides in 2 changes of 95% alcohol for 3 minutes each @ RT.
7. Wash the slides in 1 change of 80% alcohol for 3 minutes @ RT.
8. Rinse the slides in gentle running distilled water for 5 minutes @ RT.
9. Perform antigen retrieval by steaming the slides in 0.01M sodium citrate buffer (pH 6.0) @ 99-100 degrees Celcius
for 20 minutes.
10. Remove the slides from the heat and let stand in buffer @ RT for 20 minutes.
11. Rinse the slides in 1X TBS-T for 1 minute @ RT.

**Do not allow the tissues to dry at any time during the staining procedure**

12. Begin the immunostaining by applying a universal protein block for 20 minutes @ RT.
13. Drain protein block from the slides and apply the diluted primary antibody for 45 minutes @ RT.
14. Rinse the slide in 1X TBS-T for 1 minute @ RT.
15. Apply a biotinylated anti-rabbit IgG (H+L) secondary for 30 minutes @ RT.
16. Rinse the slide in 1X TBS-T for 1 minute @ RT.
17. Apply an alkaline phosphatase steptavidin for 30 minutes @ RT.
18. Rinse the slide in 1X TBS-T for 1 minute @ RT.
19. Apply an alkaline phosphatase chromagen substrate for 30 minutes @ RT.
20. Rinse the slide in distilled water for 1 minute @ RT.

**This method should only be used if the chromagen substrate is alcohol insoluble (ie: Vector Red, DAB)**

21. Dehydrate the tissue by washing the slides in 2 changes of 80% alcohol for 1 minute each @ RT.
22. Wash the slides in 2 changes of 95% alcohol for 1 minute each @ RT.
23. Wash the slides in 3 changes of 100% alcohol for 1 minute each @ RT.
24. Wash the slides in 3 changes of xylene for 1 minute each @ RT.
25. Apply cover slip.

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies