TEM5/GPR124 Antibody - BSA Free

Novus Biologicals | Catalog # NLS2167

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Porcine, Bat, Canine, Equine, Monkey, Rabbit

Predicted:

Avian (94%), Chicken (94%), Hamster (94%), Mouse (94%), Primate (100%), Rat (94%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all TEM5/GPR124 Primary Antibodies »

Product Specifications

Immunogen

Synthetic 17 amino acid peptide from N-terminal extracellular domain of human TEM5/GPR124.

Epitope

N-Terminus extracellular domain

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Gorilla (100%), Gibbon (100%), Marmoset (100%), Monkey (82%), Bovine (82%).

Localization

Membrane; multi pass membrane protein.

Specificity

Human GPR124. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except GPR125 (82%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Product can be stored undiluted at 4C for up to 1 month.

Scientific Data Images for TEM5/GPR124 Antibody - BSA Free

Immunohistochemistry-Paraffin: TEM5/GPR124 Antibody - BSA Free [NLS2167]

Immunohistochemistry-Paraffin: TEM5/GPR124 Antibody - BSA Free [NLS2167]

Immunohistochemistry-Paraffin: TEM5/GPR124 Antibody [NLS2167] - Human colon carcinoma, neoplastic cells. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Immunohistochemistry-Paraffin: TEM5/GPR124 Antibody - BSA Free [NLS2167]

Immunohistochemistry-Paraffin: TEM5/GPR124 Antibody - BSA Free [NLS2167]

Immunohistochemistry-Paraffin: TEM5/GPR124 Antibody [NLS2167] - Analysis of anti-GPR124 / TEM5 antibody with human lung, adenocarcinoma.
Immunohistochemistry-Paraffin: TEM5/GPR124 Antibody - BSA Free [NLS2167]

Immunohistochemistry-Paraffin: TEM5/GPR124 Antibody - BSA Free [NLS2167]

Immunohistochemistry-Paraffin: TEM5/GPR124 Antibody [NLS2167] - Human Skin, Kaposis Sarcoma capillary. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Immunohistochemistry-Paraffin: TEM5/GPR124 Antibody - BSA Free [NLS2167]

Immunohistochemistry-Paraffin: TEM5/GPR124 Antibody - BSA Free [NLS2167]

Immunohistochemistry-Paraffin: TEM5/GPR124 Antibody [NLS2167] - Human Prostate, Carcinoma Endothelial Cells. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Applications for TEM5/GPR124 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

4 ug/ml
Application Notes
.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

0.9 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.

Background: TEM5/GPR124

Tumor endothelial marker 5 is an Orphan-U GPCR with an unknown ligand. Tumor Endothelial Marker 5, like GPR125, has a leucine rich repeat (LRR), an immunoglobulin (Ig) domain, and a hormone-binding domain (HBD). The Ig domain shows similarities to motilin and titin, while the LRR domain shows similarities to LRIG1 and SLIT1-2. ESTs have been isolated primarily from amnion, connective tissue, ear, embryo, eye, ganglion, heart, lung, placenta, and skin libraries. TEM5 expression has been documented in endothelial cells of tumors, including colon, brain, liver. Expression is restricted largely to endothelial cells (St Croix et al. 2000).

Long Name

Tumor Endothelial Marker 5

Alternate Names

GPR124, KIAA1531

Entrez Gene IDs

25960 (Human)

Gene Symbol

ADGRA2

Additional TEM5/GPR124 Products

Product Documents for TEM5/GPR124 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for TEM5/GPR124 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

View specific protocols for TEM5/GPR124 Antibody - BSA Free (NLS2167):

Immunohistochemistry Protocol for GPR124 Antibody (NLS2167):
Immunohistochemistry

1. Prepare tissue with formalin fixation and by embedding it in paraffin wax.
2. Make 4 um sections and place on pre-cleaned and charged microscope slides.
3. Heat in a tissue-drying oven for 45 minutes @ 60 degrees Celcius.
4. Deparaffinize the tissues by wash drying the slides in 3 changes of xylene for 5 minutes each @ RT.
5. Rehydrate the tissues by washing the slides in 3 changes of 100% alcohol for 3 minutes each @ RT.
6. Wash the slides in 2 changes of 95% alcohol for 3 minutes each @ RT.
7. Wash the slides in 1 change of 80% alcohol for 3 minutes @ RT.
8. Rinse the slides in gentle running distilled water for 5 minutes @ RT.
9. Perform antigen retrieval by steaming the slides in 0.01M sodium citrate buffer (pH 6.0) @ 99-100 degrees Celcius
for 20 minutes.
10. Remove the slides from the heat and let stand in buffer @ RT for 20 minutes.
11. Rinse the slides in 1X TBS-T for 1 minute @ RT.

**Do not allow the tissues to dry at any time during the staining procedure**

12. Begin the immunostaining by applying a universal protein block for 20 minutes @ RT.
13. Drain protein block from the slides and apply the diluted primary antibody for 45 minutes @ RT.
14. Rinse the slide in 1X TBS-T for 1 minute @ RT.
15. Apply a biotinylated anti-rabbit IgG (H+L) secondary for 30 minutes @ RT.
16. Rinse the slide in 1X TBS-T for 1 minute @ RT.
17. Apply an alkaline phosphatase steptavidin for 30 minutes @ RT.
18. Rinse the slide in 1X TBS-T for 1 minute @ RT.
19. Apply an alkaline phosphatase chromagen substrate for 30 minutes @ RT.
20. Rinse the slide in distilled water for 1 minute @ RT.

**This method should only be used if the chromagen substrate is alcohol insoluble (ie: Vector Red, DAB)**

21. Dehydrate the tissue by washing the slides in 2 changes of 80% alcohol for 1 minute each @ RT.
22. Wash the slides in 2 changes of 95% alcohol for 1 minute each @ RT.
23. Wash the slides in 3 changes of 100% alcohol for 1 minute each @ RT.
24. Wash the slides in 3 changes of xylene for 1 minute each @ RT.
25. Apply cover slip.

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FAQs

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