Testosterone is an important secreted androgen synthesized from cholesterol-derived pregnenolone. Circulating testosterone is predominantly bound to proteins, including specific sex hormone binding globulin (SHBG) and nonspecific proteins such as albumin. It is believed that bioavailable testosterone includes the free steroid and the albumin-bound form. Testosterone is the main androgen secreted by the Leydig cells of the testes and affects both primary and secondary sexual development.
Testosterone Antibody (3T16) - BSA Free
Novus Biologicals | Catalog # NBP2-50332
Key Product Details
Species Reactivity
Non-species specific
Applications
ELISA, Radioimmunoassay
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 3T16
Format
BSA Free
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Product Specifications
Immunogen
Testosterone-3-(O-carboxymethyl)oxime coupled to bovine serum albumin
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Applications for Testosterone Antibody (3T16) - BSA Free
Application
Recommended Usage
ELISA
1:100 - 1:2000
Radioimmunoassay
1:100 - 1:2000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Testosterone
Alternate Names
17b-Hydroxyandrost-4-ene-3-one, 17b-Testosterone
Additional Testosterone Products
Product Documents for Testosterone Antibody (3T16) - BSA Free
Certificate of Analysis
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Product Specific Notices for Testosterone Antibody (3T16) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for Testosterone Antibody (3T16) - BSA Free
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Q: Does R&D Systems® Testosterone Parameter Assay Kit, Catalog # KGE010, detect free or total testosterone?
A: 'Free' testosterone is typically defined as testosterone which is bioavailable, and not bound to sex hormone binding globulin (SHBG). Interference testing performed by spiking SHBG into testosterone-containing samples, demonstrates interference in testosterone detection with Catalog # KGE010, when the concentration of SHBG is greater than 100 ng/ml. (For further details on interference testing, please refer to the "Specificity" section in the product insert.) Assays for 'total' testosterone measure both 'free' testostrone as well as testosterone bound to SHBG. Since KGE010 does not detect testosterone bound to SHBG, this assay does not measure 'total' testosterone.
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