Testosterone Parameter Assay Kit

(10 citations)   
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (100 uL), Serum (20 uL), EDTA Plasma (20 uL), Heparin Plasma (20 uL)
  • Sensitivity
    0.041 ng/mL
  • Assay Range
    0.0 - 10 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
  • Specificity
    Measures Testosterone levels in various samples
  • Cross-reactivity
    Cross-reactivity observed with 1 or more available related molecules.Cross-species reactivity not tested.
  • Interference
    Interference observed with 1 or more available related molecules.
Control Available
Product Summary
The Parameter Testosterone kit is a 4.5 hour competitive enzyme immunoassay designed to measure Testosterone in cell culture supernates, serum, and plasma.

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Standard Deviation0.


The recovery of Testosterone spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 103 95-117
EDTA Plasma (n=4) 94 85-107
Heparin Plasma (n=4) 92 84-101
Serum (n=4) 93 86-104
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Testosterone were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Testosterone Parameter Assay Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Testosterone
Testosterone is an important secreted androgen synthesized from cholesterol-derived pregnenolone. Circulating testosterone is predominantly bound to proteins, including specific sex hormone binding globulin (SHBG) and nonspecific proteins such as albumin. It is believed that bioavailable testosterone includes the free steroid and the albumin-bound form. Testosterone is the main androgen secreted by the Leydig cells of the testes and affects both primary and secondary sexual development.
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 50 µL Primary Antibody Solution
    4.   Add 50 µL of Primary Antibody Solution to each well (except the non-specific binding (NSB) wells).
    5.   Incubate at room temperature for 1 hour on a horizontal orbital microplate shaker.
    6.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

    7. Calibrator Diluent
    8.   Add 100 µL of Calibrator Diluent to the NSB wells.
    9.   Add 100 µL of Calibrator Diluent to the zero standard (B0) wells.

    10. 100 µL Standard, Control, or Sample
    11.   Add 100 µL of Standard, control, or sample to the remaining wells.

    12. 50 µL Conjugate
    13.   Add 50 µL of Conjugate to each well. Cover with a plate sealer, and incubate at room temperature for 3 hours on the shaker.

    14. 200 µL Substrate Solution
    15. Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.

    16. 50 µL Stop Solution
    17. Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    Showing Results 1 - 10 of 10
    Filter your results:

    Sample Type
    1. Cutting edge: c-Kit signaling differentially regulates type 2 innate lymphoid cell accumulation and susceptibility to central nervous system demyelination in male and female SJL mice.
      Authors: Russi A, Walker-Caulfield M, Ebel M, Brown M
      J Immunol, 2015;194(12):5609-13.
      Species: Mouse
      Sample Type: Serum
    2. Insulin-like 3-induced rat preantral follicular growth is mediated by growth differentiation factor 9.
      Authors: Xue, Kai, Kim, Ji Young, Liu, Jia-yin, Tsang, Benjamin
      Endocrinology, 2014;155(1):156-67.
      Species: Rat
      Sample Type: cell culture supernate
    3. Alterations of bone density, microstructure, and strength of the distal radius in male patients with rheumatoid arthritis: a case-control study with HR-pQCT.
      Authors: Zhu T, Griffith J, Qin L, Hung V, Fong T, Au S, Li M, Lam Y, Wong C, Kwok A, Leung P, Li E, Tam L
      J Bone Miner Res, 2014;29(9):2118-29.
      Species: Human
      Sample Type: Serum
    4. Endocrine responses during overnight recovery from exercise: impact of nutrition and relationships with muscle protein synthesis.
      Authors: Betts JA, Beelen M, Stokes KA, Saris W HM, van Loon L JC
      Int J Sport Nutr Exerc Metab, 2011;21(5):398-409.
      Species: Human
      Sample Type: Plasma
    5. Testosterone regulates cell proliferation in aggressive fibromatosis (desmoid tumour).
      Authors: Hong H, Nadesan P, Poon R, Alman BA
      Br. J. Cancer, 2011;104(9):1452-8.
      Species: Mouse
      Sample Type: Serum
    6. Inhibitor of DNA binding 4 is expressed selectively by single spermatogonia in the male germline and regulates the self-renewal of spermatogonial stem cells in mice.
      Authors: Oatley MJ, Kaucher AV, Racicot KE
      Biol. Reprod., 2011;85(2):347-56.
      Species: Mouse
      Sample Type: Serum
    7. Host-derived adiponectin is tumor-suppressive and a novel therapeutic target for multiple myeloma and the associated bone disease.
      Authors: Fowler JA, Lwin ST, Drake MT
      Blood, 2011;118(22):5872-82.
      Species: Human
      Sample Type: Serum
    8. Skeletal muscle myofibrillar and sarcoplasmic protein synthesis rates are affected differently by altitude-induced hypoxia in native lowlanders.
      Authors: Holm L, Haslund ML, Robach P, van Hall G, Calbet JA, Saltin B, Lundby C
      PLoS ONE, 2010;5(12):e15606.
      Species: Human
      Sample Type: Plasma
    9. Inhibition of tumor growth progression by antiandrogens and mTOR inhibitor in a Pten-deficient mouse model of prostate cancer.
      Authors: Zhang W, Zhu J, Efferson CL, Ware C, Tammam J, Angagaw M, Laskey J, Bettano KA, Kasibhatla S, Reilly JF, Sur C, Majumder PK
      Cancer Res., 2009;69(18):7466-72.
      Species: Mouse
      Sample Type: Plasma
    10. Castration Differentially Alters Basal and Leucine-Stimulated Tissue Protein Synthesis in Skeletal Muscle and Adipose Tissue.
      Authors: Jiao Q, Pruznak AM, Huber D, Vary TC, Lang CH
      Am. J. Physiol. Endocrinol. Metab., 2009;0(0):.
      Species: Rat
      Sample Type: Plasma
    ELISA Controls
    Description Application Cat# Citations Images  

    Parameter Immunoassay Control Set 891 for Testosterone

    Ctrl QC165
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    1. Does R&D Systems® Testosterone Parameter Assay Kit, Catalog # KGE010, detect free or total testosterone?

      • 'Free' testosterone is typically defined as testosterone which is bioavailable, and not bound to sex hormone binding globulin (SHBG). Interference testing performed by spiking SHBG into testosterone-containing samples, demonstrates interference in testosterone detection with Catalog # KGE010, when the concentration of SHBG is greater than 100 ng/ml. (For further details on interference testing, please refer to the "Specificity" section in the product insert.) 

        Assays for 'total' testosterone measure both 'free' testostrone as well as testosterone bound to SHBG. Since KGE010 does not detect testosterone bound to SHBG, this assay does not measure 'total' testosterone.

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