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Testosterone Parameter Assay Kit

R&D Systems | Catalog # KGE010

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Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (100 µL), Serum (20 µL), EDTA Plasma (20 µL), Heparin Plasma (20 µL)

Sensitivity

0.041 ng/mL

Assay Range

0.041-10 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
View all Testosterone ELISA Kits »

Product Summary for Testosterone Parameter Assay Kit

The Parameter Testosterone kit is a 4.5 hour competitive enzyme immunoassay designed to measure Testosterone in cell culture supernates, serum, and plasma.

Product Specifications

Format

96-well strip plate

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

Biotin

Species

Multi-Species

Specificity

Measures Testosterone levels in various samples

Cross-reactivity

Cross-reactivity observed with 1 or more available related molecules. Cross-species reactivity not tested.

Interference

Interference observed with 1 or more available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates, EDTA Plasma, Heparin Plasma, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (ng/mL) 1.25 3.50 5.80 1.26 3.67 6.03
Standard Deviation 0.05 0.11 0.17 0.07 0.23 0.41
CV% 4.0 3.1 2.9 5.6 6.3 6.8

Recovery for Testosterone Parameter Assay Kit

The recovery of Testosterone spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 103 95-117
EDTA Plasma (n=4) 94 85-107
Heparin Plasma (n=4) 92 84-101
Serum (n=4) 93 86-104

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Testosterone were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Multi-species Testosterone ELISA Linearity

Scientific Data Images for Testosterone Parameter Assay Kit

Multi-species Testosterone ELISA Standard Curve

Multi-species Testosterone ELISA Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Testosterone

Testosterone is an important secreted androgen synthesized from cholesterol-derived pregnenolone. Circulating testosterone is predominantly bound to proteins, including specific sex hormone binding globulin (SHBG) and nonspecific proteins such as albumin. It is believed that bioavailable testosterone includes the free steroid and the albumin-bound form. Testosterone is the main androgen secreted by the Leydig cells of the testes and affects both primary and secondary sexual development.

Alternate Names

17b-Hydroxyandrost-4-ene-3-one, 17b-Testosterone

Additional Testosterone Products

Product Documents for Testosterone Parameter Assay Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Testosterone Parameter Assay Kit

For research use only

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Testosterone Parameter Assay Kit

Customer Reviews for Testosterone Parameter Assay Kit (2)

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  • Testosterone Parameter Assay Kit
    Name: Anonymous
    Sample Tested: Serum
    Verified Customer | Posted 07/08/2024
    Serum samples collected by saphenous bleeding from adult C57BL6 male mice
    Testosterone Parameter Assay Kit KGE010
  • Testosterone Parameter Assay Kit
    Name: Anonymous
    Sample Tested: Serum-free Cell Culture Media and Serum
    Verified Customer | Posted 03/22/2018
    Testosterone Parameter Assay Kit KGE010

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Protocols

View specific protocols for Testosterone Parameter Assay Kit (KGE010):

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 50 µL Primary Antibody Solution
  3.   Add 50 µL of Primary Antibody Solution to each well (except the non-specific binding (NSB) wells).
  4.   Incubate at room temperature for 1 hour on a horizontal orbital microplate shaker.
  5.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
    6. Calibrator Diluent
  6.   Add 100 µL of Calibrator Diluent to the NSB wells.
  7.   Add 100 µL of Calibrator Diluent to the zero standard (B0) wells.
    8. 100 µL Standard, Control, or Sample
  8.   Add 100 µL of Standard, control, or sample to the remaining wells.
    9. 50 µL Conjugate
  9.   Add 50 µL of Conjugate to each well. Cover with a plate sealer, and incubate at room temperature for 3 hours on the shaker.
    10. 200 µL Substrate Solution
  10. Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
    11. 50 µL Stop Solution
  11. Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Testosterone Parameter Assay Kit

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  • Q: Does R&D Systems® Testosterone Parameter Assay Kit, Catalog # KGE010, detect free or total testosterone?

    A: 'Free' testosterone is typically defined as testosterone which is bioavailable, and not bound to sex hormone binding globulin (SHBG). Interference testing performed by spiking SHBG into testosterone-containing samples, demonstrates interference in testosterone detection with Catalog # KGE010, when the concentration of SHBG is greater than 100 ng/ml. (For further details on interference testing, please refer to the "Specificity" section in the product insert.) Assays for 'total' testosterone measure both 'free' testostrone as well as testosterone bound to SHBG. Since KGE010 does not detect testosterone bound to SHBG, this assay does not measure 'total' testosterone.

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