Testosterone Antibody (RM435)

Novus Biologicals | Catalog # NBP3-18821

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Non-species specific

Applications

ELISA

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # RM435 expressed in HEK293
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Product Specifications

Immunogen

Testosterone-KLH

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Testosterone Antibody (RM435)

Testosterone Antibody (RM435)

ELISA: Testosterone Antibody (RM435)[NBP3-18821]

ELISA: Testosterone Antibody (RM435)[NBP3-18821] Representative standard curve for Testosterone Competitive ELISA.

Applications for Testosterone Antibody (RM435)

Application
Recommended Usage

ELISA

1 - 10 ng

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

50% Glycerol/PBS, 1% BSA

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: Testosterone

Testosterone is an important secreted androgen synthesized from cholesterol-derived pregnenolone. Circulating testosterone is predominantly bound to proteins, including specific sex hormone binding globulin (SHBG) and nonspecific proteins such as albumin. It is believed that bioavailable testosterone includes the free steroid and the albumin-bound form. Testosterone is the main androgen secreted by the Leydig cells of the testes and affects both primary and secondary sexual development.

Alternate Names

17b-Hydroxyandrost-4-ene-3-one, 17b-Testosterone

Additional Testosterone Products

Product Documents for Testosterone Antibody (RM435)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Testosterone Antibody (RM435)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs for Testosterone Antibody (RM435)

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  • Q:

    Does R&D Systems® Testosterone Parameter Assay Kit, Catalog # KGE010, detect free or total testosterone?

    A:

    'Free' testosterone is typically defined as testosterone which is bioavailable, and not bound to sex hormone binding globulin (SHBG). Interference testing performed by spiking SHBG into testosterone-containing samples, demonstrates interference in testosterone detection with Catalog # KGE010, when the concentration of SHBG is greater than 100 ng/ml. (For further details on interference testing, please refer to the "Specificity" section in the product insert.) 

    Assays for 'total' testosterone measure both 'free' testostrone as well as testosterone bound to SHBG. Since KGE010 does not detect testosterone bound to SHBG, this assay does not measure 'total' testosterone.

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