Testosterone is an important secreted androgen synthesized from cholesterol-derived pregnenolone. Circulating testosterone is predominantly bound to proteins, including specific sex hormone binding globulin (SHBG) and nonspecific proteins such as albumin. It is believed that bioavailable testosterone includes the free steroid and the albumin-bound form. Testosterone is the main androgen secreted by the Leydig cells of the testes and affects both primary and secondary sexual development.
Sheep Testosterone ELISA Kit (Colorimetric)
Novus Biologicals | Catalog # NBP3-23574
Loading...
Key Product Details
Sample Type & Volume Required Per Well
Serum and Plasma (50 uL)
Sensitivity
0.25 ng/mL
Assay Range
0.63-40 ng/mL
Loading...
Product Specifications
Assay Type
Competitive-ELISA
Kit Type
ELISA Kit (Colorimetric)
Species
Sheep
Specificity
This kit recognizes Sheep Testosterone in samples. No significant cross-reactivity or interference between Sheep Testosterone and analogues was observed.
Precision
Intra-Assay Precision (Precision within an assay) %CV 6.71
Inter-Assay Precision (Precision between assays) %CV 6.59
Recovery for Sheep Testosterone ELISA Kit (Colorimetric)
Recovery
84-107%
Linearity
Scientific Data Images for Sheep Testosterone ELISA Kit (Colorimetric)
ELISA: Sheep Testosterone ELISA Kit (Colorimetric) [NBP3-23574] -
ELISA: Sheep Testosterone ELISA Kit (Colorimetric) [NBP3-23574] - Standard Curve ReferenceKit Contents for Sheep Testosterone ELISA Kit (Colorimetric)
- Concentrated HRP Linked Detection Ab (100x)
- Concentrated Wash Buffer (25x)
- HRP Linked Ab Diluent
- Micro ELISA Plate (Dismountable)
- Plate Sealer
- Product Manual
- Reference Standard
- Sample Diluent
- Stop Solution
- Substrate Reagent
Preparation and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Storage of components varies. See protocol for specific instructions.
Background: Testosterone
Alternate Names
17b-Hydroxyandrost-4-ene-3-one, 17b-Testosterone
Additional Testosterone Products
Product Documents for Sheep Testosterone ELISA Kit (Colorimetric)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Sheep Testosterone ELISA Kit (Colorimetric)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.
Customer Reviews for Sheep Testosterone ELISA Kit (Colorimetric)
There are currently no reviews for this product. Be the first to review Sheep Testosterone ELISA Kit (Colorimetric) and earn rewards!
Have you used Sheep Testosterone ELISA Kit (Colorimetric)?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- Troubleshooting Guide: ELISA
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for Sheep Testosterone ELISA Kit (Colorimetric)
Showing
1
-
1 of
1 FAQ
Showing All
-
Q: Does R&D Systems® Testosterone Parameter Assay Kit, Catalog # KGE010, detect free or total testosterone?
A: 'Free' testosterone is typically defined as testosterone which is bioavailable, and not bound to sex hormone binding globulin (SHBG). Interference testing performed by spiking SHBG into testosterone-containing samples, demonstrates interference in testosterone detection with Catalog # KGE010, when the concentration of SHBG is greater than 100 ng/ml. (For further details on interference testing, please refer to the "Specificity" section in the product insert.) Assays for 'total' testosterone measure both 'free' testostrone as well as testosterone bound to SHBG. Since KGE010 does not detect testosterone bound to SHBG, this assay does not measure 'total' testosterone.
Loading...