TFEB Antibody (HL1065) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-13706

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL1065 expressed in HEK293

Format

Azide and BSA Free
View all TFEB Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human TFEB. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

Centrifuge briefly prior to opening.

Scientific Data Images for TFEB Antibody (HL1065) - Azide and BSA Free

Western Blot: TFEB Antibody (HL1065) [NBP3-13706]

Western Blot: TFEB Antibody (HL1065) [NBP3-13706]

Western Blot: TFEB Antibody (HL1065) [NBP3-13706] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membranes were blotted with TFEB antibody [HL1065] (NBP3-13706) diluted at 1:5000 and competitor's antibody (# Highly Cited Antibody) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
TFEB Antibody (HL1065)

Western Blot: TFEB Antibody (HL1065) [NBP3-13706] -

Western Blot: TFEB Antibody (HL1065) [NBP3-13706] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TFEB antibody [HL1065] (NBP3-13706) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
TFEB Antibody (HL1065)

Immunohistochemistry-Paraffin: TFEB Antibody (HL1065) [NBP3-13706] -

Immunohistochemistry-Paraffin: TFEB Antibody (HL1065) [NBP3-13706] - TFEB antibody [HL1065] detects TFEB protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
TFEB stained by TFEB antibody [HL1065] (NBP3-13706) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Applications for TFEB Antibody (HL1065) - Azide and BSA Free

Application
Recommended Usage

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TFEB

Transcription factor that specifically recognizes and binds E-box sequences (3'-CANNTG-5'). Efficient DNA-binding requires dimerization with itself or with another MiT/TFE family member such as TFE3 or MITF. In association with TFE3, activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4(+) T-cells and thymus-dependent humoral immunity. Specifically recognizes and binds the CLEAR-box sequence (5'-GTCACGTGAC-3') present in the regulatory region of many lysosomal genes, leading to activate their expression. It thereby plays a central role in expression of lysosomal genes. Specifically recognizes the gamma-E3 box, a subset of E-boxes, present in the heavy-chain immunoglobulin enhancer. Plays a role in the signal transduction processes required for normal vascularization of the placenta

Long Name

Transcription Factor EB

Alternate Names

ALPHATFEB, BHLHE35, TCFEB

Gene Symbol

TFEB

Additional TFEB Products

Product Documents for TFEB Antibody (HL1065) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for TFEB Antibody (HL1065) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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