Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all TFIIIC Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1475 and 1525 of human general transcription factor IIIC, polypeptide 1 (general transcription factor IIIC, 220kDa subunit) using the numbering given in entry NP_001511.2 (GeneID 2

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TFIIIC Antibody - BSA Free

Immunohistochemistry: TFIIIC Antibody [NB100-60656]

Immunohistochemistry: TFIIIC Antibody [NB100-60656]

Immunohistochemistry: TFIIIC Antibody [NB100-60656] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-GTF3C1/TFIIIC220 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunohistochemistry-Paraffin: TFIIIC Antibody [NB100-60656]

Immunohistochemistry-Paraffin: TFIIIC Antibody [NB100-60656]

Immunohistochemistry-Paraffin: TFIIIC Antibody [NB100-60656] - FFPE section of human thyroid carcinoma. Affinity purified rabbit anti-TFIIIC used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit
Immunohistochemistry-Paraffin: TFIIIC Antibody [NB100-60656]

Immunohistochemistry-Paraffin: TFIIIC Antibody [NB100-60656]

Immunohistochemistry-Paraffin: TFIIIC Antibody [NB100-60656] - FFPE section of human breast carcinoma. Affinity purified rabbit anti-TFIIIC used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit
Immunohistochemistry-Paraffin: TFIIIC Antibody [NB100-60656]

Immunohistochemistry-Paraffin: TFIIIC Antibody [NB100-60656]

Immunohistochemistry-Paraffin: TFIIIC Antibody [NB100-60656] - FFPE section of human prostate carcinoma. Affinity purified rabbit anti-TFIIIC used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit.
Immunoprecipitation: TFIIIC Antibody [NB100-60656]

Immunoprecipitation: TFIIIC Antibody [NB100-60656]

Immunoprecipitation: TFIIIC Antibody [NB100-60656] - Detection of Human TFIIIC on HeLa whole cell lysate using NB100-60656. TFIIIC was efficiently IPed by rabbit anti-TFIIIC antibody NB100-60655 but not by NB100-60657.

Applications for TFIIIC Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:500- 1:2000

Immunohistochemistry-Paraffin

1:100-1:500

Immunoprecipitation

2-5 ug/mg lysate
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: TFIIIC

General transcription factor 3C polypeptide 1 (GTF3C1/TFIIIC220) is a subunit of the TFIIIC DNA-binding factor required for transcription of tRNA and 5S rRNA genes by RNA polymerase III. Human TFIIIC is composed of six subunits: TFIIIC220, TFIIIC110, TFIIIC102, TFIIIC90 and TFIIIC63, and TFIIIC35. The functional contribution of GTF3C1/TFIIIC220 to the TFIIIC complex has not been fully determined.

Alternate Names

general transcription factor 3C polypeptide 1, general transcription factor IIIC, polypeptide 1 (alpha subunit), general transcription factor IIIC, polypeptide 1 (alpha subunit, 220kD ), general transcription factor IIIC, polypeptide 1, alpha 220kDa, TF3C-alpha, TFIIIC, TFIIIC 220 kDa subunit, TFIIIC box B-binding subunit, TFIIIC220DKFZp686A111, TFIIICalpha, Transcription factor IIIC 220 kDa subunit, Transcription factor IIIC subunit alpha

Entrez Gene IDs

2975 (Human)

Gene Symbol

GTF3C1

UniProt

NP_001511.2 (Human)

Additional TFIIIC Products

Product Documents for TFIIIC Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for TFIIIC Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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