Loading...
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Peptide ELISA
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Loading...
Product Specifications
Immunogen
Peptide with sequence C-VKIAYEEIFVKNM corresponding to C-Terminus according to NP_006278.1.
Specificity
This antibody is expected to recognize isoform a (NP_006278.1) only.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for TFPI Antibody
Western Blot: TFPI Antibody [NB100-786]
Western Blot: TFPI Antibody [NB100-786] - Western blot analysis of TFPI in Hela lysate using NB100-786 at 0.05 ug/ ml.Western Blot: TFPI Antibody [NB100-786]
Western Blot: TFPI Antibody [NB100-786] - HEK293 overexpressing TFPI and probed with (mock transfection in first lane).Applications for TFPI Antibody
Application
Recommended Usage
Peptide ELISA
Detection limit 1:64000
Western Blot
0.3 - 1 ug/ml
Application Notes
WB: Approx. 50 kDa band observed in lysates of HepG2 and HeLa (calculated MW of 35.0 kDa band according to NP_006278.1). In transfected HEK293 transiently expressing TFPI bands of approx. 38 kDa band to 45 kDa band are observed. These bands are not observed in the non-transfected HEK293.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: TFPI
Long Name
Tissue Factor Pathway Inhibitor
Alternate Names
EPI, LACI
Entrez Gene IDs
7035 (Human)
Gene Symbol
TFPI
UniProt
Additional TFPI Products
Product Documents for TFPI Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for TFPI Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Customer Reviews for TFPI Antibody
There are currently no reviews for this product. Be the first to review TFPI Antibody and earn rewards!
Have you used TFPI Antibody?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...