Tie-1 Antibody (6F12) - Azide and BSA Free

Novus Biologicals | Catalog # NBP1-18610

Novus Biologicals

Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 6F12

Format

Azide and BSA Free
View all Tie-1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant human soluble extracellular TIE-1

Specificity

The monoclonal antibody will detect native human TIE-1 in ELISA experiments and on the surface of different human cell types.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

Novus Biologicals Mouse Tie-1 Antibody (6F12) - Azide and BSA Free (NBP1-18610) is a monoclonal antibody validated for use in WB, ELISA and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Tie-1 Antibody (6F12) - Azide and BSA Free

Western Blot: Tie-1 Antibody (6F12)Azide and BSA Free [NBP1-18610]

Western Blot: Tie-1 Antibody (6F12)Azide and BSA Free [NBP1-18610]

Western Blot: Tie-1 Antibody (Cl. 6F12) [NBP1-18610] - Analysis of recombinant human and mouse soluble TIEreceptors using a monoclonal antibody directed against human recombinant soluble TIE-1. There is a weak cross reactivity with human sTIE-2 but no reactivity with mouse sTIE-1 and sTIE-2 visible.
Flow Cytometry: Tie-1 Antibody (6F12) - Azide and BSA Free [NBP1-18610]

Flow Cytometry: Tie-1 Antibody (6F12) - Azide and BSA Free [NBP1-18610]

Flow Cytometry: Tie-1 Antibody (Cl. 6F12) [NBP1-18610] - Fig. 1: Flow analysis of TIE-1 expression in primary human umbilical vein endothelial cells (HUVEC).

Applications for Tie-1 Antibody (6F12) - Azide and BSA Free

Application
Recommended Usage

ELISA

1 - 15 ug/ml

Western Blot

1 - 2 ug/ml
Application Notes
This antibody is Cytof ready.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein G purified

Reconstitution

Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/ml. Please note the sample size of this product will be provided in reconstituted liquid form.

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Tie-1

TIE1/TIE (tyrosine kinase with Ig and EGF homology domains 1) and TIE2/Tek define a new class of the receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin like domains flanking three epidermal growth factor (EFG) like domains followed by three fibronectin type III like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region. Human TIE1 cDNA encodes a 1138 amino acid residue precursor protein with a putative signal peptide, an extracellular domain, and a cytoplasmic domain. Human TIE1/Fc, a disulfide linked homodimeric protein, has a calculated molecular mass of approximately 107 kDa. Due to glycosylation, the protein migrates to approximately 160 kDa in SDS PAGE under reducing conditions. TIE1 and TIE2, expressed primarily on endothelial and hematopoietic progenitor cells, play important roles in angiogenesis, vasculogenesis, and hematopoiesis. In developing vascular endothelial cells, TIE1 and TIE2 are specifically expressed. Two ligands that bind TIE have been identified, angiopoietin 1 and angiopoietin 2. Based on gene targeting studies, the in vivo functions of TIE1 are related to endothelial cell differentiation. The receptor tyrosine kinase TIE also plays a role in the survival and integrity of the endothelium.

Long Name

Tyrosine Kinase with Immunoglobulin and Epidermal Growth Factor Homology Domains 1

Alternate Names

Tie1

Entrez Gene IDs

7075 (Human)

Gene Symbol

TIE1

UniProt

P35590

Additional Tie-1 Products

Product Documents for Tie-1 Antibody (6F12) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for Tie-1 Antibody (6F12) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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