TIRAP Antibody

Novus Biologicals | Catalog # NB300-990

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Western Blot, Peptide ELISA, Proximity Ligation Assay

Cited:

Proximity Ligation Assay

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all TIRAP Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-EGEGERDSATVSDL corresponding to C-Terminus according to NP_683708.1.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 30579042).

Specificity

This protein is expected to recognise isoform b (NP_683708.1) only.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for TIRAP Antibody

Western Blot: TIRAP Antibody [NB300-990]

Western Blot: TIRAP Antibody [NB300-990]

Western Blot: TIRAP (TLR2 and TLR4) Antibody [NB300-990] - (1 ug/ml) staining of human liver lysate 35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for TIRAP Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:128000

Western Blot

1 - 3 ug/ml
Application Notes
WB: Approx. 30 kDa band observed in human liver lysates (calculated MW of 25.3 kDa band according to NP_683708.1). Use in Proximity ligation assay reported in scientific literature (PMID: 30579042).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TIRAP

The innate immune system recognizes microbial pathogens through Toll-like receptors (TLRs), which identify pathogen-associated molecular patterns. Different TLRs recognize different pathogen-associated molecular patterns and all TLRs have a Toll-interleukin 1 receptor (TIR) domain, which is responsible for signal transduction. The protein encoded by this gene is a TIR adaptor protein involved in the TLR4 signaling pathway of the immune system. It activates NF-kappa-B, MAPK1, MAPK3 and JNK, which then results in cytokine secretion and the inflammatory response. Alternative splicing of this gene results in several transcript variants; however, not all variants have been fully described.

Alternate Names

adapter protein wyatt, Adaptor protein Wyatt, MAL, MyD88 adapter-like protein, MyD88-2, TIR domain-containing adapter protein, toll/interleukin-1 receptor domain-containing adapter protein, toll-interleukin 1 receptor (TIR) domain containing adaptor protein, Toll-interleukin 1 receptor (TIR) domain-containing adaptor protein, Toll-like receptor adaptor protein, wyatt

Entrez Gene IDs

114609 (Human)

Gene Symbol

TIRAP

UniProt

NP_683708. (Human)

Additional TIRAP Products

Product Documents for TIRAP Antibody

Certificate of Analysis

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Product Specific Notices for TIRAP Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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