TMPRSS4 Antibody

Novus Biologicals | Catalog # NBP1-36997

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Chimpanzee (%), Monkey (%). Backed by our 100% Guarantee.

Applications

Western Blot, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all TMPRSS4 Primary Antibodies »

Product Specifications

Immunogen

TMPRSS4 Antibody is made to a peptide with sequence HCFRKHTDVFNWK corresponding to internal region according to NP_063947.1, NP_001077416.1.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Chimpanzee (100%), Gibbon (100%), Marmoset (100%), Monkey (100%)

Specificity

TMPRSS4 Antibody is expected to recognise both reported isoforms (NP_063947.1 and NP_001077416.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for TMPRSS4 Antibody

Western Blot: TMPRSS4 Antibody [NBP1-36997]

Western Blot: TMPRSS4 Antibody [NBP1-36997]

Western Blot: TMPRSS4 Antibody [NBP1-36997] - Analysis of TMPRSS4 in HEK293 lysate (35ug protein in RIPA buffer) using this antibody at 1ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence. The observed molecular weight is represented by the band appearing at 48 kDa.

Applications for TMPRSS4 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:1000

Western Blot

0.3 - 1 ug/ml
Application Notes
WB: Approx. 48 kDa band observed in lysates of HEK293 and A549 (calculated MW of 48.2 kDa band according to NP_063947.1).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TMPRSS4

TMPRSS4, previously known as TMPRSS3, is a 437 amino acid type II transmembrane serine protease located on human chromosome 11q23.3 that encodes 5 isoforms containing a complete coding sequence. This serine protease has a predicted molecular weight of 48 kDa with 2 glycosylation sites and the cleaved soluble protease domain has been detected in cell culture media. Functions of TMPRSS4 include embryo development, viral infection, and cancer. TMPRSS4 plays a role in the epithelial-mesenchymal transition (EMT) process and mediates cell invasion, migration, proliferation and metastasis. Overexpression of TMPRSS4 has been reported for multiple solid tumors including pancreatic, ovarian, thyroid, colorectal, lung, breast, cervical, gallbladder, gastric, and liver cancer, and has been associated with poor overall survival and reduced time to tumor progression (1,2).
TMPRSS4 has also been shown to play a role in the process of viral entry for coronaviruses and influenza viruses thru proteolytic activation of key viral glycoproteins. One pathway for SARS-CoV-2 infection, which causes the COVID-19 disease, involves binding of the SARS-CoV-2 Spike 'S' glycoprotein to the human ACE2 receptor and cleavage of S by TMPRSS2 or potentially by TMPRSS4 at the cell surface to facilitate viral entry (3).
References

1. L de Aberasturi A, Calvo A. (2015) TMPRSS4: An Emerging Potential Therapeutic Target in Cancer. Br J Cancer. 112(1):4-8. PMID: 25203520

2. Zeng P., Zhang P., Zhou L., Tang M., Shen Y., Jin J., Zhu Y., Chen M. (2016) TMPRSS4 as an emerging potential poor prognostic factor for solid tumors: A systematic review and meta-analysis. Oncotarget. 7:76327-76336. PMID: 27344186

3. Zang F, Castro MFG, McCune BT, Zeng Q, Rothlauf PW, Sonnek NM, Liu Z, Brulois KF, Wang X, Greenberg HB, Diamond MS, Ciorba MA, Whelan SPJ, and Ding S. (2020) TMPRSS2 and TMPRSS4 promote SARS-CoV-2 infection of human small intestinal enterocytes. Sci Immunol. 5(47): eabc3582. PMID: 32404436

Long Name

Transmembrane protease, serine 4

Alternate Names

CAPH2, MT-SP2

Entrez Gene IDs

56649 (Human)

Gene Symbol

TMPRSS4

UniProt

Q9NRS4 (Human)

Additional TMPRSS4 Products

Product Documents for TMPRSS4 Antibody

Certificate of Analysis

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Product Specific Notices for TMPRSS4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Serine Proteases and Regulators

Citations for TMPRSS4 Antibody

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