TOP2A Antibody (rTOP2A/8339)

Novus Biologicals | Catalog # NBP3-48998

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG1 kappa Clone # rTOP2A/8339 expressed in HEK293
View all TOP2A Primary Antibodies »

Product Specifications

Immunogen

Recombinant human Topoisomerase II alpha fragment (aa1300-1500) (exact sequence is proprietary) (Uniprot: P11388)

Localization

Nucleus

Specificity

It recognizes a 170kDa protein, which is identified as topoisomerase II. It shows no cross-reaction with Topoisomerase II or I.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

170 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Scientific Data Images for TOP2A Antibody (rTOP2A/8339)

TOP2A Antibody (rTOP2A/8339)

Immunohistochemistry-Paraffin: TOP2A Antibody (rTOP2A/8339) [NBP3-48998] -

Formalin-fixed, paraffin-embedded human tonsil stained with TOP2A Antibody (rTOP2A/8339). Inset: PBS instead of primary antibody; secondary only negative control.

Applications for TOP2A Antibody (rTOP2A/8339)

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: TOP2A

Topo II Alpha encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromsome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia.

Long Name

DNA Topoisomerase II alpha

Alternate Names

TOP2, TP2A

Gene Symbol

TOP2A

Additional TOP2A Products

Product Documents for TOP2A Antibody (rTOP2A/8339)

Certificate of Analysis

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Product Specific Notices for TOP2A Antibody (rTOP2A/8339)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers

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Protocols

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FAQs

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