Topoisomerase I Antibody

Novus Biologicals | Catalog # NBP1-30481

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Topoisomerase I Primary Antibodies »

Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 100 and 150 of human DNA topoisomerase I using the numbering given in entry NP_003277.1 (GeneID 7150).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

91 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Topoisomerase I Antibody

Western Blot: Topoisomerase I Antibody [NBP1-30481]

Western Blot: Topoisomerase I Antibody [NBP1-30481]

Western Blot: Topoisomerase I Antibody [NBP1-30481] - Whole cell lysate from HeLa and 293T cells. TOP1 was less efficiently immunoprecipitated by rabbit anti-TOP1 antibody NBP1-30482.

Applications for Topoisomerase I Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Western blot of lysates performed using standard western blot reagents and 4-8% SDS-PAGE.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Topoisomerase I

Topoisomerases are nuclear enzymes involved in a variety of cellular activities such as chromosome condensation, DNA replication, transcription, recombination and segregation at mitosis. Human topoisomerase I is a 100kD protein capable of relaxing positively and negatively supercoiled DNA by performing a transient single stranded nick which is then religated at the end of the reaction. It has been shown that the enzyme is located in regions of the genome that are undergoing active RNA synthesis, where it probably reduces superhelical stresses in the DNA, enabling RNA polymerase to function properly. Both DNA topoisomerases I and II have been found to be targets of autoantibodies in the sera of patients with certain autoimmune diseases such as systemic lupus erythematosus and also of some anti tumor drugs and antibiotics. Elevated levels of DNA topoisomerase I, detected by transfer assays, have been demonstrated in colorectal tumors compared with normal colon mucosa as a result of increased transcription or mRNA stability.

Long Name

DNA topoisomerase 1

Alternate Names

DNA topoisomerase I, EC 5.6.2.1, TOP1

Entrez Gene IDs

7150 (Human)

Gene Symbol

TOP1

UniProt

P11387 (Human)

Additional Topoisomerase I Products

Product Documents for Topoisomerase I Antibody

Certificate of Analysis

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Download SDS

Product Specific Notices for Topoisomerase I Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Topoisomerase I Antibody

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FAQs for Topoisomerase I Antibody

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  • Q: Do you have any data about the reactivity of this antibody against mouse Top1. I want to use this antibody for both mouse and human Top1. Your description says it is reactive against human Top1. Will it also equally work against Mouse Top1?

    A: Since the immunogen of NBP1-30481 maps to a region between residues 100-150 of human DNA topoisomerase I, based on the sequence identity, I would not recommend it for mouse samples. I did a blast search on the immunogen and unfortunately there is not much homology with mouse Top1.

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