Total Nitric Oxide and Nitrate/Nitrite Parameter Assay Kit

(60 citations)
(1 Review)
  
  • Format
    96-well strip plate
  • Assay Length
    < 1 hour
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (10 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL), Citrate Plasma (25 uL), Urine (10 uL)
  • Sensitivity
    0.78 umol/L
  • Assay Range
    3.1 - 200 umol/L (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma, Urine)
  • Specificity
    Measures Total Nitric Oxide, Nitrite and Nitrate levels in various samples
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    Interference observed with other substances.
Control Available
QC135, Parameter Immunoassay Control Set 904 for Nitrite - Please Inquire
QC134, Parameter Immunoassay Control Set 849 for Nitrate - Please Inquire
Product Summary
The Parameter Total Nitric Oxide kit has two assay options. Endogenous nitrite is measured in the first option. In the second option, nitrate is converted to nitrite using nitrate reductase and total nitrite is measured. To obtain the nitrate concentration, endogenous nitrite is subtracted from the total nitrite value.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma, Urine
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020404040
Mean3077.213831.579.9138.7
Standard Deviation0.761.12.21.462.914.78
CV%2.51.41.64.63.63.4

Recovery

The recovery of the nitrate standard spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (Nitrite Assay) (n=10) 107 91-115
Plasma (Nitrate Assay) (n=25) 98 87-110
Plasma (Nitrite Assay) (n=20) 104 92-117
Serum (Nitrate Assay) (n=10) 95 89-101
Serum (Nitrite Assay) (n=10) 109 98-118
Urine (Nitrite Assay) (n=10) 101 87-112
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of the nitrate standard were serially diluted with the Reaction Diluent to produce samples with values within the dynamic range of the assay.
Total Nitric Oxide and Nitrate/Nitrite Parameter Assay Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
  • Alternate Names
    Nitric Oxide;
Related Research Areas
Assay Procedure
Nitrite Assay Procedure
Nitrate Reduction Assay Procedure


Refer to the product for complete assay procedure.

This assay procedure measures the concentration of endogenous nitrite present in the sample. Bring all reagents and samples to room temperature before use. It is recommended that all samples and standards be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 50 µL Reaction Diluent (1X)
  4.   Add 50 µL of Reaction Diluent (1X) to the Blank wells.

  5. 50 µL Nitrite Standard or Sample
  6.   Add 50 µL of Nitrite Standard or sample to the remaining wells.

  7. 50 µL Reaction Diluent (1X)
  8.   Add 50 µL of Reaction Diluent (1X) to all wells.

  9. 50 µL Griess Reagent I
  10.   Add 50 µL of Griess Reagent I to all wells.

  11. 50 µL Griess Reagent II
  12.   Add 50 µL of Griess Reagent II to all wells. Mix by gently tapping the side of the plate.
  13.   Incubate at room temperature for 10 minutes. Read at 540 nm with wavelength correction at 690 nm.



Refer to the product for complete assay procedure.

This assay procedure measures total nitrite by converting nitrate to nitrite. To determine the nitrate concentration in the sample, the endogenous nitrite concentration measured from the Nitrite Assay Procedure must be subtracted from the converted nitrite concentration measured in this procedure. Bring all reagents and samples to room temperature before use. It is recommended that all samples and standards be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 50 µL Reaction Diluent (1X)
  4.   Add 50 µL of Reaction Diluent (1X) to the Blank wells.

  5. 50 µL Nitrate Standard or Sample
  6.   Add 50 µL of Nitrate Standard or sample to the remaining wells.

  7. 25 µL NADH
  8.   Add 25 µL of NADH to all wells.

  9. 25 µL Nitrate Reductase
  10.   Add 25 µL of diluted Nitrate Reductase to all wells. Mix well, cover with a plate sealer, and incubate at 37 °C for 30 minutes.

  11. 50 µL Griess Reagent I
  12.   Add 50 µL of Griess Reagent I to all wells.

  13. 50 µL Griess Reagent II
  14.   Add 50 µL of Griess Reagent II to all wells. Mix by gently tapping the side of the plate.
  15.   Incubate at room temperature for 10 minutes. Read at 540 nm with wavelength correction at 690 nm.
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

60 Citations: Showing 1 - 10
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Species
Sample Type
  1. Cynanchum wilfordii Polysaccharides Suppress Dextran Sulfate Sodium-Induced Acute Colitis in Mice and the Production of Inflammatory Mediators from Macrophages
    Authors: CW Cho, S Ahn, TG Lim, HD Hong, YK Rhee, DC Yang, M Jang
    Mediators Inflamm., 2017;2017(0):3859856.
    Species: Mouse
    Sample Type: Cell Culture Supernates
  2. Defective Platelet Activation and Bleeding Complications upon Cholestasis in Mice
    Authors: NS Gowert, M Klier, M Reich, F Reusswig, L Donner, V Keitel, D Häussinger, M Elvers
    Cell. Physiol. Biochem., 2017;41(6):2133-2149.
    Species: Mouse
    Sample Type: Plasma
  3. Exosomes from eosinophils autoregulate and promote eosinophil functions
    Authors: JA Ca¤as, B Sastre, C Mazzeo, M Fern ndez-, JM Rodrigo-Mu, A Gonz lez-G, M Izquierdo, P Barranco, S Quirce, J Sastre, V Del Pozo
    J. Leukoc. Biol, 2017;0(0):.
    Species: Human
    Sample Type: Cell Culture Supernates
  4. Inflammatory Mediators in Tracheal Aspirates of Preterm Infants Participating in a Randomized Trial of Inhaled Nitric Oxide
    Authors: M Laube, E Amann, U Uhlig, Y Yang, HW Fuchs, M Zemlin, JC Mercier, RF Maier, HD Hummler, S Uhlig, UH Thome
    PLoS ONE, 2017;12(1):e0169352.
    Species: Human
    Sample Type: Tissue Secretion
  5. Serelaxin as a potential treatment for renal dysfunction in cirrhosis: Preclinical evaluation and results of a randomized phase 2 trial
    Authors: VK Snowdon, NJ Lachlan, AM Hoy, PW Hadoke, SI Semple, D Patel, W Mungall, TJ Kendall, A Thomson, RJ Lennen, MA Jansen, CM Moran, A Pellicoro, P Ramachandr, I Shaw, RL Aucott, T Severin, R Saini, J Pak, D Yates, N Dongre, JS Duffield, DJ Webb, JP Iredale, PC Hayes, JA Fallowfiel
    PLoS Med, 2017;14(2):e1002248.
    Species: Human
    Sample Type: Serum
  6. Nitric Oxide Synthase Trafficking Inducer is a pleiotropic regulator of endothelial cell function and signaling
    Authors: S Chakrabort, R Ain
    J. Biol. Chem, 2017;0(0):.
  7. Cerium Oxide Nanoparticles in Lung Acutely Induce Oxidative Stress, Inflammation, and DNA Damage in Various Organs of Mice
    Authors: A Nemmar, P Yuvaraju, S Beegam, MA Fahim, BH Ali
    Oxid Med Cell Longev, 2017;2017(0):9639035.
    Species: Mouse
    Sample Type: Tissue Homogenates
  8. Histological and Immunohistochemical Basis of the Effect of Aminoguanidine on Renal Changes Associated with Hemorrhagic Shock in a Rat Model
    Authors: A Al Drees, M Salah Khal, M Soliman
    Acta Histochem Cytochem, 2017;50(1):11-19.
    Species: Rat
    Sample Type: Plasma
  9. Toll-like receptor polymorphisms compromise the inflammatory response against bacterial antigen translocation in cirrhosis
    Authors: P Piñero, O Juanola, E Caparrós, P Zapater, P Giménez, JM González-N, J Such, R Francés
    Sci Rep, 2017;7(0):46425.
    Species: Human
    Sample Type: Serum
  10. Elucidation of Molecular Mechanisms of Streptozotocin-Induced Oxidative Stress, Apoptosis, and Mitochondrial Dysfunction in Rin-5F Pancreatic ?-Cells
    Authors: AMTA Nahdi, A John, H Raza
    Oxid Med Cell Longev, 2017;2017(0):7054272.
    Species: Rat
    Sample Type: Cell Culture Supernates
  11. Prostate cancer downregulated SIRP-? modulates apoptosis and proliferation through p38-MAPK/NF-?B/COX-2 signaling
    Authors: C Yao, G Li, M Cai, Y Qian, L Wang, L Xiao, F Thaiss, B Shi
    Oncol Lett, 2017;13(6):4995-5001.
    Species: Human
    Sample Type: Cell Culture Supernates
  12. Benzo[a]pyrene activates interleukin-6 induction and suppresses nitric oxide-induced apoptosis in rat vascular smooth muscle cells
    Authors: HP Tzeng, KC Lan, TH Yang, MN Chung, SH Liu
    PLoS ONE, 2017;12(5):e0178063.
    Species: Rat
    Sample Type: Cell Culture Supernates
  13. Danhong Huayu Koufuye Prevents Diabetic Retinopathy in Streptozotocin-Induced Diabetic Rats via Antioxidation and Anti-Inflammation
    Authors: W Chen, X Yao, C Zhou, Z Zhang, G Gui, B Lin
    Mediators Inflamm., 2017;2017(0):3059763.
    Species: Rat
    Sample Type: Serum
  14. The anti-inflammatory and immunomodulatory potential of braylin: Pharmacological properties and mechanisms by in silico, in vitro and in vivo approaches
    Authors: RF Espírito-S, CS Meira, RDS Costa, OP Souza Filh, AF Evangelist, GHG Trossini, GM Ferreira, EDS Velozo, CF Villarreal, MB Pereira So
    PLoS ONE, 2017;12(6):e0179174.
    Species: Mouse
    Sample Type: Cell Culture Supernates
  15. Aberrant Inflammasome Activation Characterizes Tuberculosis-Associated Immune Reconstitution Inflammatory Syndrome
    Authors: HY Tan, YK Yong, EM Shankar, G Paukovics, R Ellegård, M Larsson, A Kamarulzam, MA French, SM Crowe
    J Immunol, 2016;0(0):.
    Species: Human
    Sample Type: Plasma
  16. Controlled Release of Interleukin-1 Receptor Antagonist from Hyaluronic Acid-Chitosan Microspheres Attenuates Interleukin-1?-Induced Inflammation and Apoptosis in Chondrocytes
    Biomed Res Int, 2016;2016(0):6290957.
    Species: Rat
    Sample Type: Cell Culture Supernates
  17. Irisin Lowers Blood Pressure by Improvement of Endothelial Dysfunction via AMPK-Akt-eNOS-NO Pathway in the Spontaneously Hypertensive Rat
    J Am Heart Assoc, 2016;5(11):.
    Species: Human
    Sample Type: Cell Culture Supernates
  18. Revisiting the expression and function of follicle-stimulation hormone receptor in human umbilical vein endothelial cells
    Sci Rep, 2016;6(0):37095.
    Species: Human
    Sample Type: Cell Culture Supernates
  19. Progesterone Attenuates Microglial-Driven Retinal Degeneration and Stimulates Protective Fractalkine-CX3CR1 Signaling
    PLoS ONE, 2016;11(11):e0165197.
    Species: Mouse
    Sample Type: Cell Culture Supernates
  20. Physicochemistry and cardiovascular toxicity of metal fume PM2.5: a study of human coronary artery endothelial cells and welding workers
    Sci Rep, 2016;6(0):33515.
    Species: Human
    Sample Type: Cell Culture Supernates
  21. Impact of Genetic Polymorphism of methylenetetrahydrofolate reductase C677T on Development of Hyperhomocysteinemia and Related Oxidative Changes in Egyptian ?-Thalassemia Major Patients
    PLoS ONE, 2016;11(5):e0155070.
    Species: Human
    Sample Type: Plasma
  22. The homeoprotein DLX4 controls inducible nitric oxide synthase-mediated angiogenesis in ovarian cancer.
    Authors: Trinh B, Ko S, Haria D, Barengo N, Naora H
    Mol Cancer, 2015;14(0):97.
    Species: Human
    Sample Type: Cell Culture Supernates
  23. Vasculopathy-associated hyperangiotensinemia mobilizes haematopoietic stem cells/progenitors through endothelial AT(2)R and cytoskeletal dysregulation.
    Authors: Chang K, Nayak R, Roy S, Perumbeti A, Wellendorf A, Bezold K, Pirman M, Hill S, Starnes J, Loberg A, Zhou X, Inagami T, Zheng Y, Malik P, Cancelas J
    Nat Commun, 2015;6(0):5914.
    Species: Mouse
    Sample Type: Plasma
  24. Estradiol promotes M1-like macrophage activation through cadherin-11 to aggravate temporomandibular joint inflammation in rats.
    Authors: Kou X, Li C, He D, Wang X, Hao T, Meng Z, Zhou Y, Gan Y
    J Immunol, 2015;194(6):2810-8.
    Species: Rat
    Sample Type: Cell Culture Supernates
  25. High levels of pigment epithelium-derived factor in diabetes impair wound healing through suppression of Wnt signaling.
    Authors: Qi W, Yang C, Dai Z, Che D, Feng J, Mao Y, Cheng R, Wang Z, He X, Zhou T, Gu X, Yan L, Yang X, Ma J, Gao G
    Diabetes, 2015;64(4):1407-19.
    Species: Mouse
    Sample Type: Plasma
  26. Direct Renin Inhibition with Aliskiren Improves Ischemia-Induced Neovasculogenesis in Diabetic Animals via the SDF-1 Related Mechanism.
    Authors: Chang T, Wu T, Huang P, Lin C, Chen J, Lin L, Lin S, Chen J
    PLoS ONE, 2015;10(8):e0136627.
    Species: Mouse
    Sample Type: Plasma
  27. Aspirin treatment improved mesenchymal stem cell immunomodulatory properties via the 15d-PGJ2/PPARgamma/TGF-beta1 pathway.
    Authors: Tang J, Xiong J, Wu T, Tang Z, Ding G, Zhang C, Wang S, Liu Y
    Stem Cells Dev, 2014;23(17):2093-103.
    Species: Mouse
    Sample Type: Cell Culture Supernates
  28. An airway epithelial iNOS-DUOX2-thyroid peroxidase metabolome drives Th1/Th2 nitrative stress in human severe asthma.
    Authors: Voraphani N, Gladwin M, Contreras A, Kaminski N, Tedrow J, Milosevic J, Bleecker E, Meyers D, Ray A, Ray P, Erzurum S, Busse W, Zhao J, Trudeau J, Wenzel S
    Mucosal Immunol, 2014;7(5):1175-85.
    Species: Human
    Sample Type: Cell Culture Supernates
  29. Renal hemodynamic effect of sodium-glucose cotransporter 2 inhibition in patients with type 1 diabetes mellitus.
    Authors: Cherney D, Perkins B, Soleymanlou N, Maione M, Lai V, Lee A, Fagan N, Woerle H, Johansen O, Broedl U, von Eynatten M
    Circulation, 2014;129(5):587-97.
    Species: Human
    Sample Type: Plasma
  30. Telomerase governs immunomodulatory properties of mesenchymal stem cells by regulating FAS ligand expression.
    Authors: Chen C, Akiyama K, Yamaza T, You Y, Xu X, Li B, Zhao Y, Shi S
    EMBO Mol Med, 2014;6(3):322-34.
    Species: Human
    Sample Type: Cell Culture Supernates
  31. Effects of pitavastatin versus atorvastatin on the peripheral endothelial progenitor cells and vascular endothelial growth factor in high-risk patients: a pilot prospective, double-blind, randomized study.
    Authors: Lin L, Huang C, Chen J, Wu T, Leu H, Huang P, Chang T, Lin S, Chen J
    Cardiovasc Diabetol, 2014;13(0):111.
    Species: Human
    Sample Type: Serum
  32. Increase in the mediators of asthma in obesity and obesity with type 2 diabetes: reduction with weight loss.
    Authors: Dandona P, Ghanim H, Monte S, Caruana J, Green K, Abuaysheh S, Lohano T, Schentag J, Dhindsa S, Chaudhuri A
    Obesity (Silver Spring), 2014;22(2):356-62.
    Species: Human
    Sample Type: Plasma
  33. Lung protective ventilation induces immunotolerance and nitric oxide metabolites in porcine experimental postoperative sepsis.
    Authors: Sperber J, Lipcsey M, Larsson A, Larsson A, Sjolin J, Castegren M
    PLoS ONE, 2013;8(12):e83182.
    Species: Porcine
    Sample Type: Urine
  34. Modulation of inflammatory response in a cirrhotic rat model with induced bacterial peritonitis.
    Authors: Sanchez E, Frances R, Soriano G, Mirelis B, Sancho F, Gonzalez-Navajas J, Munoz C, Song X, Perez-Mateo M, Such J, Guarner C
    PLoS ONE, 2013;8(3):e59692.
    Species: Rat
    Sample Type: Serum
  35. Comparison of drug and cell-based delivery: engineered adult mesenchymal stem cells expressing soluble tumor necrosis factor receptor II prevent arthritis in mouse and rat animal models.
    Authors: Liu L, Wang G, Hendricks K, Lee K, Bohnlein E, Junker U, Mosca J
    Stem Cells Transl Med, 2013;2(5):362-75.
    Species: Human
    Sample Type: Cell Culture Supernates
  36. Increased intrahepatic resistance in severe steatosis: endothelial dysfunction, vasoconstrictor overproduction and altered microvascular architecture.
    Authors: Francque S, Laleman W, Verbeke L, Van Steenkiste C, Casteleyn C, Kwanten W, Van Dyck C
    Lab Invest, 2012;92(10):1428-39.
    Species: Rat
    Sample Type: Serum
  37. Nitric oxide-producing myeloid-derived suppressor cells inhibit vascular E-selectin expression in human squamous cell carcinomas.
    J. Invest. Dermatol., 2012;132(11):2642-51.
    Species: Human
    Sample Type: Cell Culture Supernates
  38. CD4 T cells promote rather than control tuberculosis in the absence of PD-1-mediated inhibition.
    Authors: Barber DL, Mayer-Barber KD, Feng CG
    J. Immunol., 2011;186(3):1598-607.
    Species: Mouse
    Sample Type: BALF
  39. The C5a receptor impairs IL-12-dependent clearance of Porphyromonas gingivalis and is required for induction of periodontal bone loss.
    Authors: Liang S, Krauss JL, Domon H, McIntosh ML, Hosur KB, Qu H, Li F, Tzekou A, Lambris JD, Hajishengallis G
    J. Immunol., 2011;186(2):869-77.
    Species: Mouse
    Sample Type: Cell Culture Supernates
  40. Caspase-1 independent IL-1beta production is critical for host resistance to mycobacterium tuberculosis and does not require TLR signaling in vivo.
    Authors: Mayer-Barber KD, Barber DL, Shenderov K, White SD, Wilson MS, Cheever A, Kugler D, Hieny S, Caspar P, Nunez G, Schlueter D, Flavell RA, Sutterwala FS, Sher A
    J. Immunol., 2010;184(7):3326-30.
    Species: Mouse
    Sample Type: BALF
  41. Molecular adsorbent recirculating system is ineffective in the management of type 1 hepatorenal syndrome in patients with cirrhosis with ascites who have failed vasoconstrictor treatment.
    Authors: Wong F, Raina N, Richardson R
    Gut, 2010;59(3):381-6.
    Species: Human
    Sample Type: Plasma
  42. Loss of ability to self-heal malaria upon taurine transporter deletion.
    Authors: Delic D, Warskulat U, Borsch E, Al-Qahtani S, Al-Quraishi S, Haussinger D, Wunderlich F
    Infect. Immun., 2010;78(4):1642-9.
    Species: Mouse
    Sample Type: Serum
  43. Chrysanthemum indicum Linne extract inhibits the inflammatory response by suppressing NF-kappaB and MAPKs activation in lipopolysaccharide-induced RAW 264.7 macrophages.
    Authors: Cheon MS, Yoon T, Lee do Y, Choi G, Moon BC, Lee AY, Choo BK, Kim HK
    J Ethnopharmacol, 2009;122(3):473-7.
    Species: Mouse
    Sample Type: Cell Culture Supernates
  44. Nox2-containing NADPH oxidase deficiency confers protection from hindlimb ischemia in conditions of increased oxidative stress.
    Authors: Haddad P, Dussault S, Groleau J, Turgeon J, Michaud SE, Menard C, Perez G, Maingrette F, Rivard A
    Arterioscler. Thromb. Vasc. Biol., 2009;29(10):1522-8.
    Species: Mouse
    Sample Type: Plasma
  45. Endothelial nitric oxide synthase gene polymorphisms and Plasmodium falciparum infection in Indian adults.
    Authors: Dhangadamajhi G, Mohapatra BN, Kar SK, Ranjit M
    Infect. Immun., 2009;77(7):2943-7.
    Species: Human
    Sample Type: Plasma
  46. Decreased nitric oxide products in the urine of patients undergoing cardiac surgery.
    Authors: Lema G, Urzua J, Jalil R, Canessa R, Vogel A, Moran S, Fajuri A, Carvajal C, Aeschlimann N, Jaque MP
    J. Cardiothorac. Vasc. Anesth., 2009;23(2):188-94.
    Species: Human
    Sample Type: Urine
  47. Uropathogenic Escherichia coli-induced inflammation alters mouse urinary bladder contraction via an interleukin-6-activated inducible nitric oxide synthase-related pathway.
    Authors: Weng TI, Wu HY, Lin PY, Liu SH
    Infect. Immun., 2009;77(8):3312-9.
    Species: Mouse
    Sample Type: Tissue Homogenates
  48. Aerobic interval training reduces cardiovascular risk factors more than a multitreatment approach in overweight adolescents.
    Authors: Tjonna AE, Stolen TO, Bye A, Volden M, Slordahl SA, Odegard R, Skogvoll E, Wisloff U
    Clin. Sci., 2009;116(4):317-26.
    Species: Human
    Sample Type: Plasma
  49. Impaired sodium excretion and increased blood pressure in mice with targeted deletion of renal epithelial insulin receptor.
    Authors: Tiwari S, Sharma N, Gill PS, Igarashi P, Kahn CR, Wade JB, Ecelbarger CM
    Proc. Natl. Acad. Sci. U.S.A., 2008;105(17):6469-74.
    Species: Mouse
    Sample Type: Urine
  50. Acute onset human atrial fibrillation is associated with local cardiac platelet activation and endothelial dysfunction.
    Authors: Akar JG, Jeske W, Wilber DJ
    J. Am. Coll. Cardiol., 2008;51(18):1790-3.
    Species: Human
    Sample Type: Plasma
  51. Leptin, blood pressure, and aerobic capacity in women.
    Authors: Sabatier MJ, McCully KK, Marinik EL, Schwark EH, Haddow S, Cortez-Cooper M, Bergeron MF, Sloan GJ, Cannon JG
    Am. J. Hypertens., 2008;21(11):1245-50.
    Species: Human
    Sample Type: Serum
  52. Reversal of axonal loss and disability in a mouse model of progressive multiple sclerosis.
    Authors: Basso AS, Frenkel D, Quintana FJ, Costa-Pinto FA, Petrovic-Stojkovic S, Puckett L, Monsonego A, Bar-Shir A, Engel Y, Gozin M, Weiner HL
    J. Clin. Invest., 2008;118(4):1532-43.
    Species: Mouse
    Sample Type: Cell Culture Supernates
  53. Pathogen induction of CXCR4/TLR2 cross-talk impairs host defense function.
    Authors: Hajishengallis G, Wang M, Liang S, Triantafilou M, Triantafilou K
    Proc. Natl. Acad. Sci. U.S.A., 2008;105(36):13532-7.
    Species: Mouse
    Sample Type: Peritoneal Fluid
  54. Particulate matter exposure induces persistent lung inflammation and endothelial dysfunction.
    Authors: Tamagawa E, Bai N, Morimoto K, Gray C, Mui T, Yatera K, Zhang X, Xing L, Li Y, Laher I, Sin DD, Man SF, van Eeden SF
    Am. J. Physiol. Lung Cell Mol. Physiol., 2008;295(1):L79-85.
    Species: Rabbit
    Sample Type: Serum
  55. Aerobic interval training versus continuous moderate exercise as a treatment for the metabolic syndrome: a pilot study.
    Authors: Tjonna AE, Lee SJ, Rognmo Ø, Stolen TO, Bye A, Haram PM, Loennechen JP, Al-Share QY, Skogvoll E, Slordahl SA, Kemi OJ, Najjar SM, Wisloff U
    Circulation, 2008;118(4):346-54.
    Species: Human
    Sample Type: Plasma
  56. Effects of atorvastatin therapy on hypercholesterolemic rabbits with respect to oxidative stress, nitric oxide pathway and homocysteine.
    Authors: Bolayirli IM, Aslan M, Balci H, Altug T, Hacibekiroglu M, Seven A
    Life Sci., 2007;81(2):121-7.
    Species: Rabbit
    Sample Type: Serum
  57. The role of caveolin-1 in PCB77-induced eNOS phosphorylation in human-derived endothelial cells.
    Authors: Lim EJ, Smart EJ, Toborek M, Hennig B
    Am. J. Physiol. Heart Circ. Physiol., 2007;293(6):H3340-7.
    Species: Porcine
    Sample Type: Cell Culture Supernates
  58. Role of extracellular signal-regulated kinases (ERK) in leptin-induced hypertension.
    Authors: Wojcicka G, Jamroz-Wisniewska A, Widomska S, Ksiazek M, Beltowski J
    Life Sci., 2007;82(7):402-12.
    Species: Rat
    Sample Type: Plasma
  59. Retinal vascular endothelial growth factor induces intercellular adhesion molecule-1 and endothelial nitric oxide synthase expression and initiates early diabetic retinal leukocyte adhesion in vivo.
    Authors: Joussen AM, Poulaki V, Qin W, Kirchhof B, Mitsiades N, Wiegand SJ, Rudge J, Yancopoulos GD, Adamis AP
    Am. J. Pathol., 2002;160(2):501-9.
    Species: Rat
    Sample Type: Tissue Homogenates
  60. Endothelial Cell Response to Fusobacterium nucleatum.
    Authors: Mendes R, Pamuk F
    Infect Immun, 0;84(7):2141-8.
    Species: Human
    Sample Type: Cell Culture Supernates
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 Total Nitric Oxide and Nitrate/Nitrite Parameter Assay Kit KGE001
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 Total Nitric Oxide and Nitrate/Nitrite Parameter Assay Kit KGE001
: Total Nitric Oxide and Nitrate/Nitrite Parameter Assay Kit [KGE001]

Summary

Sample TestedHuman serum

Other Experimental Details

Other Experimental DetailsI did spike in study for both nitrite and nitrate assay. The recovery of Nitrite is very good. However the nitrate is very poor.

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