Transglutaminase 2/TGM2 Antibody (CUB7402)

Novus Biologicals | Catalog # NBP2-26458

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human, Guinea Pig

Cited:

Mouse, Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin

Cited:

Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CUB7402
View all Transglutaminase 2/TGM2 Primary Antibodies »

Product Specifications

Immunogen

BALB/C mice were injected with purified guinea pig liver TGase II.

Localization

Cytoplasmic, cell membrane, extracellular matrix

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Transglutaminase 2/TGM2 Antibody (CUB7402)

Immunohistochemistry-Paraffin: Transglutaminase 2/TGM2 Antibody (CUB7402) [NBP2-26458]

Immunohistochemistry-Paraffin: Transglutaminase 2/TGM2 Antibody (CUB7402) [NBP2-26458]

Immunohistochemistry-Paraffin: Transglutaminase 2/TGM2 Antibody (CUB7402) [NBP2-26458] - Formalin fixed paraffin embedded breast carcinoma stained with Transglutaminase 2/TGM2 antibody (NBP2-26458).
Transglutaminase 2/TGM2 Antibody (CUB7402)

Western Blot: Transglutaminase 2/TGM2 Antibody (CUB7402) [NBP2-26458] -

Manipulating the expression of Zbtb7a in WT and TG2−/− astrocytes differentially impacts morphology. (a) Immunoblots showing knockdown (KD) or overexpression (OE) of Zbtb7a, compared to control (CTL), in WT and TG2−/− astrocytes. Since the Zbtb7a OE virus encodes human Zbtb7a, the Zbtb7a bands in these samples migrated slightly faster than bands in the CTL and KD samples from mouse astrocytes. (b) Representative images of the astrocyte GFAP network in the different conditions (scale bar = 20 um) and (c) Quantitation of GFAP network area (n = 34–50 astrocytes per condition from two independent biological replicates, Two-way ANOVA * p < 0.05, *** p < 0.001). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37047031), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Transglutaminase 2/TGM2 Antibody (CUB7402)

Application
Recommended Usage

Immunohistochemistry

1:50-1:75

Immunohistochemistry-Paraffin

1:50-1:75
Application Notes
IHC-P: recommended pretreatment of citrate buffer, pH 6.0. Recommended incubation time of 30 min at RT.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS (pH 7.4), 0.2% BSA, Tween-20

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Transglutaminase 2/TGM2

Principal Names: Transglutaminase 2 (C polypeptide, protein-glutamine-gamma-glutamyltransferase) Official Gene Symbol: TGM2 Gene ID: 7052 (Human) Gene Map Locus: 20q12 (Human). Tissue type transglutaminase (TGase II) is historically a member of the transglutaminase family that catalyzes a calcium-dependent acyl transfer reaction between peptide bound glutamine and a suitable primary amine. Transglutaminase has been implicated to play parts in diverse biologic processes such as cell proliferation, deposition and stabilization of the fine structure of the extracellular matrix and in wound repair and fibrosis. TGase II is an effector protein during cell death. The protein is induced by retinoic acid and is the autoantigen implicated in celiac disease. Transcript variants encoding three different isoforms have been found for this gene.

Alternate Names

TGC, TGM2, Transglutaminase 2, tTG

Gene Symbol

TGM2

Additional Transglutaminase 2/TGM2 Products

Product Documents for Transglutaminase 2/TGM2 Antibody (CUB7402)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Transglutaminase 2/TGM2 Antibody (CUB7402)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Transglutaminase 2/TGM2 Antibody (CUB7402)

Customer Reviews for Transglutaminase 2/TGM2 Antibody (CUB7402)

There are currently no reviews for this product. Be the first to review Transglutaminase 2/TGM2 Antibody (CUB7402) and earn rewards!

Have you used Transglutaminase 2/TGM2 Antibody (CUB7402)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies