TRIM16 Antibody (5F4) - Azide and BSA Free

Novus Biologicals | Catalog # H00010626-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Sandwich ELISA, Simple Western, Immunoprecipitation, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 5F4

Format

Azide and BSA Free
View all TRIM16 Primary Antibodies »

Product Specifications

Immunogen

TRIM16 (NP_006461, 165 a.a. ~ 273 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. LDAARRDKEAELQCTQLDLERKLKLNENAISRLQANQKSVLVSVSEVKAVAEMQFGELLAAVRKAQANVMLFLEEKEQAALSQANGIKAHLEYRSAEMEKSKQELERMA

Reactivity Notes

Human. Other species not tested.

Specificity

TRIM16 - tripartite motif-containing 16

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for TRIM16 Antibody (5F4) - Azide and BSA Free

Western Blot: TRIM16 Antibody (5F4) [H00010626-M01]

Western Blot: TRIM16 Antibody (5F4) [H00010626-M01]

Western Blot: TRIM16 Antibody (5F4) [H00010626-M01] - Analysis of TRIM16 expression in transfected 293T cell line by TRIM16 monoclonal antibody (M01), clone 5F4.Lane 1: TRIM16 transfected lysate(64 KDa).Lane 2: Non-transfected lysate.
Western Blot: TRIM16 Antibody (5F4) [H00010626-M01]

Western Blot: TRIM16 Antibody (5F4) [H00010626-M01]

Western Blot: TRIM16 Antibody (5F4) [H00010626-M01] - Analysis of TRIM16 over-expressed 293 cell line, cotransfected with TRIM16 Validated Chimera RNAi ( Cat # H00010626-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with TRIM16 monoclonal antibody (M01), clone 5F4 (Cat # H00010626-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Immunoprecipitation: TRIM16 Antibody (5F4) [H00010626-M01]

Immunoprecipitation: TRIM16 Antibody (5F4) [H00010626-M01]

Immunoprecipitation: TRIM16 Antibody (5F4) [H00010626-M01] - Analysis of TRIM16 transfected lysate using anti-TRIM16 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with TRIM16 MaxPab rabbit polyclonal antibody.
Sandwich ELISA: TRIM16 Antibody (5F4) [H00010626-M01] - Detection limit for recombinant GST tagged TRIM16 is approximately 0.03 ng/mL as a capture antibody.
Simple Western: TRIM16 Antibody (5F4) [H00010626-M01]

Simple Western: TRIM16 Antibody (5F4) [H00010626-M01]

Simple Western: TRIM16 Antibody (5F4) [H00010626-M01] - This antibody (1:25 dilution) was used to probe THP-1 macrophage lysate (concentrations shown). Simple Western image submitted by a verified customer review.

Applications for TRIM16 Antibody (5F4) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against recombinant protein for Western blot. It has also been used for RNAi validation and ELISA. This TRIM16 Antibody (5F4) is validated for Simple Western from a verified customer review.
See Simple Western Antibody Database for Simple Western validation: Tested in THP-1 macrophage lysate 0.2 mg/mL, 0.6 mg/mL and 1.2 mg/mL, separated by Size, antibody dilution of 1:25

Reviewed Applications

Read 1 review rated 3 using H00010626-M01 in the following applications:

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: TRIM16

This gene was identified as an estrogen and anti-estrogen regulated gene in epithelial cells stably expressing estrogen receptor. The protein encoded by this gene contains two B box domains and a coiled-coiled region that are characteristic of the B box zinc finger protein family. The proteins of this family have been reported to be involved in a variety of biological processes including cell growth, differentiation and pathogenesis. Expression of this gene was detected in most tissues. Its function, however, has not yet been determined.

Alternate Names

DKFZp434O1826, EBBPtripartite motif-containing 16, Estrogen-responsive B box protein, tripartite motif containing 16, tripartite motif-containing protein 16

Entrez Gene IDs

10626 (Human)

Gene Symbol

TRIM16

OMIM

609505 (Human)

UniProt

NP_006461 (Human)

Additional TRIM16 Products

Product Documents for TRIM16 Antibody (5F4) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for TRIM16 Antibody (5F4) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for TRIM16 Antibody (5F4) - Azide and BSA Free (1)

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  • TRIM16 Antibody (5F4)
    Name: Anonymous
    Application: Simple Western
    Sample Tested: THP-1 macrophage lysate
    Species: Human
    Verified Customer | Posted 12/05/2019
    This antibody (1:25 dilution) was used to probe THP-1 macrophage lysate (concentrations shown) by Simple Western.
    TRIM16 Antibody (5F4) - Azide and BSA Free H00010626-M01

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