TRIM2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-81504

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
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Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: KASLQVQLDAVNKRLPEIDSALQFISEIIHQLTNQKASIVDDIHSTFDELQKTLNVRKSVLLMELEVNYGLKHK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for TRIM2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: TRIM2 Antibody [NBP1-81504]

Immunocytochemistry/ Immunofluorescence: TRIM2 Antibody [NBP1-81504]

Immunocytochemistry/Immunofluorescence: TRIM2 Antibody [NBP1-81504] - Staining of human cell line U-251 MG shows localization to centrosome. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: TRIM2 Antibody [NBP1-81504]

Immunohistochemistry-Paraffin: TRIM2 Antibody [NBP1-81504]

Staining of human kidney shows moderate cytoplasmic positivity in cells in distal tubules.
Immunohistochemistry-Paraffin: TRIM2 Antibody [NBP1-81504]

Immunohistochemistry-Paraffin: TRIM2 Antibody [NBP1-81504]

Staining of human cerebral cortex shows strong cytoplasmic positivity in glial cells.
Immunohistochemistry-Paraffin: TRIM2 Antibody [NBP1-81504]

Immunohistochemistry-Paraffin: TRIM2 Antibody [NBP1-81504]

Staining of human lymph node shows no positivity in non-germinal center cells as expected.
TRIM2 Antibody - BSA Free Immunohistochemistry-Paraffin: TRIM2 Antibody [NBP1-81504]

Immunohistochemistry-Paraffin: TRIM2 Antibody [NBP1-81504]

Staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.

Applications for TRIM2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

Use in Western Blot reported in scientific literature (PMID:30916596).
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TRIM2

TRIM2 is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic filaments. Its function has not been identified.

Alternate Names

KIAA0517tripartite motif protein 2, RING finger protein 86, RNF86tripartite motif protein TRIM2, tripartite motif containing 2, tripartite motif-containing 2, tripartite motif-containing protein 2

Gene Symbol

TRIM2

Additional TRIM2 Products

Product Documents for TRIM2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for TRIM2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for TRIM2 Antibody - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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