Q: Can this kit (NBP2-60606) be used with urine samples?

A: Urine samples have a very low level but some sample is measurable. There is no disease sample to verify. Most normal urine samples are higher than blank.

Human Triosephosphate isomerase ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP2-60606

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Product Specifications
Precision
Recovery
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Sample Type & Volume Required Per Well

Serum, Plasma, Cell Culture (50 uL)

Sensitivity

0.17 ng/mL (example only; lot dependent)

Assay Range

0.313 - 20 ng/mL (example only; lot dependent)

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Product Specifications

Assay Type

Sandwich ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Human

Description

This assay employs a quantitative enzyme immunoassay technique that measures the specified antigen in samples.

Precision

Intra-Assay Precision (Precision within an assay) %CV 5.3% (example only; lot dependent)

Inter-Assay Precision (Precision between assays) %CV 11.6% (example only; lot dependent)

Recovery for Human Triosephosphate isomerase ELISA Kit (Colorimetric)

Recovery

90 - 111% (example only; lot dependent)

Scientific Data Images for Human Triosephosphate isomerase ELISA Kit (Colorimetric)

ELISA: Human Triosephosphate isomerase ELISA Kit (Colorimetric) [NBP2-60606]

ELISA: Human Triosephosphate isomerase ELISA Kit (Colorimetric) [NBP2-60606] - Standard curve.

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Triosephosphate isomerase

Triosephosphate isomerase (TIM) catalyses the reversible interconversion of G3P and DHAP. Only G3P can be used in glycolysis, therefore TIM is essential for energy production, allowing two molecules of G3P to be produced for every glucose molecule, thereby doubling the energy yield. Defects in TPI1 are the cause of triosephosphate isomerase deficiency (TPI deficiency) [MIM:190450]. TPI deficiency is an autosomal recessive disorder. It is the most severe clinical disorder of glycolysis. It is associated with neonatal jaundice, chronic hemolytic anemia, progressive neuromuscular dysfunction, cardiomyopathy and increased susceptibility to infection.

Alternate Names

EC 5.3.1.1, MGC88108, TIM, TPI, triosephosphate isomerase, Triose-phosphate isomerase, triosephosphate isomerase 1

Gene Symbol

TPI1

Additional Triosephosphate isomerase Products

Product Documents for Human Triosephosphate isomerase ELISA Kit (Colorimetric)

Download Product Datasheets

Download Manual for NBP2-60606 Human Triosephosphate isomerase ELISA Kit (Colorimetric)

Certificate of Analysis

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Product Specific Notices for Human Triosephosphate isomerase ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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