Q: I am searching (with difficulty) for an antibody against Trka which can be used in flow cytometry. Since I am planning to use it for bead sorting of live cells it needs to be for a part of the receptor which is on the outside of the cell. Please can you let me know if you can help me with this.

A: NBP1-47436, NB100-98815 and NB100-65266 all bind to the extracellular domain of Trka, however, none of them are guaranteed for use in flow cytometry. Our only flow cytometry guaranteed antibodies bind to the intracellular domain. This does not mean one of these antibodies won't work for flow, we just can not guarantee it. If you would be interested in testing this novel application on one of our Trka antibodies, please take a look at our Innovators Reward Program.

TrkA Antibody Pair [HRP]

Novus Biologicals | Catalog # NBP2-79341

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Product Specifications
Scientific Data
Kit Contents
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Assay Type

Sandwich ELISA

Assay Range

156.25-10000 pg/ml (example only; lot dependent)

Sensitivity

156.25 pg/ml (example only; lot dependent)

Reactivity

Human

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Product Specifications

Description

Solid Phase sandwich ELISA for the quantitative determination of Human TrkA.

Sample Volume Required

100 ul

Conjugate

HRP

Scientific Data Images for TrkA Antibody Pair [HRP]

Sandwich ELISA: TrkA Antibody Pair [HRP] [NBP2-79341] - This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.

Kit Contents for TrkA Antibody Pair [HRP]

Mouse Monoclonal Capture Antibody
Rabbit Polyclonal Detection Antibody (HRP-conjugated)
Standard

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: TrkA

The Trk proto-oncogene encodes a 140 kDa, membrane-spanning protein tyrosine kinase that is expressed only in neural tissues. Nerve growth factor (NGF) stimulates phosphorylation of Trk A in neural cell lines and in embryonic dorsal root ganglia. Affinity cross-linking and equilibrium binding experiments with 125I-labeled NGF indicate that Trk A binds NGF specifically in cultured cells with a dissociation constant of 10(-9) molar. The identification of Trk A as an NGF receptor indicates that this protein participates in the primary signal transduction mechanism of NGF (1). Trk A was found to be expressed in the nervous system and phosphorylated in response to NGF (Nerve Growth Factor). Somatic rearrangement(s) of the TRKA gene (also designated NTRK1) are responsible for formation of some oncogenes (2). Trk A is expressed in neural and nonneuronal tissues. Like RET, Trk A is often activated by rearrangements that involve one of at least five other genes in papillary thyroid carcinoma (PTC) (3).

Long Name

Neurotrophic Tyrosine Kinase Receptor A

Alternate Names

NTRK-1, NTRK1

Gene Symbol

NTRK1

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Product Documents for TrkA Antibody Pair [HRP]

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Certificate of Analysis

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Product Specific Notices for TrkA Antibody Pair [HRP]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Antibody Pairs are guaranteed for 6 months from date of receipt.

Related Research Areas

Receptor Tyrosine Kinases (RTKs)

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