Troponin I type 2 (fast skeletal) Antibody
Novus Biologicals | Catalog # NBP2-26201
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Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot, Peptide ELISA
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
Peptide with sequence C-EVRVQKTSKELED corresponding to internal region according to NP_003273.1, NP_001139313.1.
Specificity
This antibody is expected to recognize both reported isoforms (NP_003273.1; NP_001139313.1). Reported variants represent identical protein: NP_001139301.1, NP_003273.1.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Troponin I type 2 (fast skeletal) Antibody
Western Blot: Troponin I type 2 (fast skeletal) Antibody [NBP2-26201]
Western Blot: Troponin I type 2 (fast skeletal) Antibody [NBP2-26201] - Staining of Human (A), Mouse(B) and Rat (C) Skeletal Muscle lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.Applications for Troponin I type 2 (fast skeletal) Antibody
Application
Recommended Usage
Peptide ELISA
Detection limit 1:8000
Western Blot
1 - 3 ug/ml
Application Notes
WB: Approx. 25 kDa band observed in human, mouse and rat skeletal muscle lysates (calculated MW of 21.3 kDa band according to NP_003273.1).
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Troponin I type 2 (fast skeletal)
Alternate Names
skeletal, fast, troponin I type 2 (skeletal, fast), troponin I, fast skeletal muscle, Troponin I, fast-twitch isoform, troponin I, fast-twitch skeletal muscle isoform
Entrez Gene IDs
7136 (Rat)
Gene Symbol
TNNI2
Additional Troponin I type 2 (fast skeletal) Products
Product Documents for Troponin I type 2 (fast skeletal) Antibody
Certificate of Analysis
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Product Specific Notices for Troponin I type 2 (fast skeletal) Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for Troponin I type 2 (fast skeletal) Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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