Key Product Details

Species Reactivity

Human, Porcine, Bovine (Negative), Canine (Negative), Feline (Negative), Fish (Negative), Goat (Negative), Mouse (Negative), Rabbit (Negative), Rat (Negative)

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 22B11

Format

BSA Free
View all Troponin I/TNNI3 Primary Antibodies »

Product Specifications

Immunogen

Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with cTnI.

Epitope

a.a.r. 20-24

Reactivity Notes

Cross-reacts with Human and Pig. Does not cross-react with Mouse, Rat, Cow, Cat, Dog, Fish, Goat or Rabbit. Not yet tested in other species.

Specificity

This detects the unphosphorylated form of cardiac troponin I.No cross-reaction with mono or biphosphorylated troponin I.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Troponin I Type 3 (cardiac) Antibody (22B11) - BSA Free

Western Blot: Troponin I Type 3 (cardiac) Antibody (22B11) [NB120-8290]

Western Blot: Troponin I Type 3 (cardiac) Antibody (22B11) [NB120-8290]

Western Blot: Troponin I (cardiac) Antibody (22B11)[NB120-8290]-Momoclonal antibodies 22B11(A), 10B11(B), and 8E10(C) recognizing dephospho- and phospho- forms of human cardiac Tnl.

Applications for Troponin I Type 3 (cardiac) Antibody (22B11) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Western Blot

1:100-1:2000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Troponin I/TNNI3

Troponin I is part of a heteromeric complex playing an important role in the regulation of skeletal and cardiac muscle contraction. It consists of three subunits, troponin I (TnI), troponin T (TnT) and troponin C (TnC). Each subunit is responsible for part of troponin complex function. TnI inhibits ATPase activity of acto myosin and TnT and TnI are present in cardiac muscles in different forms than in skeletal muscles. Only one tissue specific isoform of TnI is described for cardiac muscle tissue (cTnI) and this is expressed only in myocardium. This antibody reacts with subunit I of the troponin complex in human myocardium, where it is the only troponin I isotype present. It does not cross-react with troponin I of skeletal muscle cells.

Long Name

Troponin I3, Cardiac Type

Alternate Names

CMD2A, CTnI, RCM1, TNNC1, TNNI3,

Entrez Gene IDs

7137 (Human); 21955 (Mouse); 171409 (Rat)

Gene Symbol

TNNI3

Additional Troponin I/TNNI3 Products

Product Documents for Troponin I Type 3 (cardiac) Antibody (22B11) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Troponin I Type 3 (cardiac) Antibody (22B11) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs for Troponin I Type 3 (cardiac) Antibody (22B11) - BSA Free

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  • Q: Would you be able to share the protocol used to validate NB120-8290 – TnI mAb (22B11) in WB?

    A:

    The western blot image on our site was submitted to us a while ago via a customer review. From the notes I have, they ran their western blot very similar to our general protocol. However, they used 5% nonfat milk with 1% BSA in TBST for blocking and diluents.

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