TROVE2 Overexpression Lysate

Name: TROVE2 Overexpression Lysate
Brand: Novus Biologicals
SKU: NBP2-09806
Price: 689 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-09806

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Product Summary
Product Specifications
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species

Human

Applications

Western Blot

Product Summary for TROVE2 Overexpression Lysate

TROVE2 Transient Overexpression Lysate

Expression Host: HEK293T

Plasmid: RC221780

Accession#: NM_004600

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.

Product Specifications

Application Notes

This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.

Theoretical MW

60.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Type

Overexpression

Scientific Data Images for TROVE2 Overexpression Lysate

Western Blot: TROVE2 Overexpression Lysate [NBP2-09806]

Western Blot: TROVE2 Overexpression Lysate (Adult Normal) [NBP2-09806] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TROVE2.

Formulation, Preparation, and Storage

Formulation

RIPA buffer

Concentration

The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.

Shipping

The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Storage

Store at -80C. Avoid freeze-thaw cycles.

Background: TROVE2

RNA-binding protein that binds to several small cytoplasmic RNA molecules known as Y RNAs. May stabilize these RNAs from degradation

Alternate Names

60 kDa ribonucleoprotein Ro, 60 kDa Ro protein, 60 kDa SS-A/Ro ribonucleoprotein, Ro 60 kDa autoantigen, RO60gastric cancer multi-drug resistance protein, RORNP, Sjoegren syndrome antigen A2, Sjoegren syndrome type A antigen, Sjogren syndrome antigen A2 (60kDa, ribonucleoprotein autoantigen SS-A/Ro), SS-A, SSA2Sjogren syndrome antigen A2 (60kD, ribonucleoprotein autoantigen SS-A/Ro), TROVE domain family member 2, TROVE domain family, member 2

Gene Symbol

RO60

Additional TROVE2 Products

Product Documents for TROVE2 Overexpression Lysate

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Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for TROVE2 Overexpression Lysate

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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