TRPC6 Antibody

Novus Biologicals | Catalog # NB600-791

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Porcine (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Peptide ELISA, Flow Cytometry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all TRPC6 Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-EKLSMEPNQEETN corresponding to C-Terminus according to NP_004612.2.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for TRPC6 Antibody

Western Blot: TRPC6 Antibody [NB600-791]

Western Blot: TRPC6 Antibody [NB600-791]

Western Blot: TRPC6 Antibody [NB600-791] - Staining of A549 cell lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.
Flow Cytometry: TRPC6 Antibody [NB600-791]

Flow Cytometry: TRPC6 Antibody [NB600-791]

Flow Cytometry: TRPC6 Antibody [NB600-791] - Analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Applications for TRPC6 Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/mL

Peptide ELISA

Detection limit 1:2000

Western Blot

0.3 - 1 ug/mL
Application Notes
Western blot: Approx 100 kDa band observed in lysates of cell line A549 (calculated MW of 106 kDa according to NP_004612.2). Preliminary experiments also showed 100 kDa in Human Lung lysates and in lysates of cell line HEK293.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: TRPC6

The protein encoded by the TRPC6 gene creates receptor-activated cation permeant calcium channels in the cell membrane. Isoform 1 is 931 amino acids in length and is approximately 106 kDa, while isoform 2 is 815 amino acids in lenth at around 93 kDA. Isoform 3 is 876 amino acids long and is just over 100 kDA. Focal segmental glomerulosclerosis 2 (FSGS2) is caused by deficits in the TRPC6 gene. This gene has been linked to diseases such as cystic fibrosis, gigantism, hypertension, kidney disease, glomerulosclerosis, pyloric stenosis, diencephalic neoplasm, premature ovarian failure, and hepatopulmonary syndrome. The TRPC6 gene interacts with MX1, ORAI1, NPHS1, NPHS2, and FYN genes in pathways that monitor sodium as well as calcium channels, EPO signaling pathway, G alpha (q) signaling events, signal transduction, and platelet homeostasis.

Long Name

Short transient receptor potential channel 6

Alternate Names

FSGS2, TRP-6, TRP6

Entrez Gene IDs

7225 (Human); 22068 (Mouse)

Gene Symbol

TRPC6

UniProt

NP_004612.2 (Human)

Additional TRPC6 Products

Product Documents for TRPC6 Antibody

Certificate of Analysis

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Product Specific Notices for TRPC6 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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