TRPM7 [p Ser1569] Antibody

Novus Biologicals | Catalog # NBP3-23192

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all TRPM7 Primary Antibodies »

Product Specifications

Immunogen

Phospho-TRPM7 (Ser-1569) synthetic peptide (coupled to carrier) corresponding to amino acids surrounding Ser-1569 in human TRPM7. This site is well conserved in rat and mouse TRPM7, and is conserved in TRPM6 (Thr-1724) however the site is threonine instead of serine. This site has no homology to other TRPM family members. [UniProt# Q96QT4]

Modification

p Ser1569

Specificity

The antibody was cross-adsorbed to unphosphorylated TRPM7 (Ser-1569) then affinity purified using phospho-TRPM7 (Ser-1569) peptide. This antibody detects a 220 kDa protein on SDS-PAGE immunoblots of human MDA-MB-231 stimulated with calyculin A and rat PC12 cells, as well as detects a 70 kDa autophosphorylated recombinant kinase domain of human TRPM7. These reactivities are not observed after lambda phosphatase treatment to dephosphorylate TRPM7.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

220 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Stable for at least 1 year at -20°C.

Scientific Data Images for TRPM7 [p Ser1569] Antibody

TRPM7 [p Ser1569] Antibody

Western Blot: TRPM7 [p Ser1569] Antibody [NBP3-23192] -

Western Blot: TRPM7 [p Ser1569] Antibody [NBP3-23192] - Western blot image of rat PC12 cells (lanes 1-4). The blot was treated with lambda phosphatase to dephosphorylate TRPM7 (lanes 2 & 4). The blot was probed with rabbit polyclonals anti-TRPM7 (Extracellular region) NBP3-23361 (lanes 1 & 2) or anti-TRPM7 (Ser-1569), phospho-specific (lanes 3 & 4).

Applications for TRPM7 [p Ser1569] Antibody

Application
Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1 mg/ml BSA and 50% glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: TRPM7

The TRPM7 (Transient Receptor Protein-PhosphoLipase C-Interacting Kinase) protein is a member of the ion channel proteins. It regulates the calcium concentration in cells, which is imperative for such functions as muscle contractions and neuronal firing. TRPM7, however, is unique among the TRP channel proteins because it is bi-functional. It not only controls ion channels but it also acts as a kinase by phosphorylating proteins.

Alternate Names

CHAK, CHAK1FLJ25718, Channel-kinase 1, EC 2.7.11.1, Long transient receptor potential channel 7, LTRPC ion channel family member 7, LTrpC7, LTrpC-7, LTRPC7FLJ20117, transient receptor potential cation channel subfamily M member 7, transient receptor potential cation channel, subfamily M, member 7, transient receptor potential-phospholipase C-interacting kinase, TRP-PLIK

Gene Symbol

TRPM7

Additional TRPM7 Products

Product Documents for TRPM7 [p Ser1569] Antibody

Certificate of Analysis

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Product Specific Notices for TRPM7 [p Ser1569] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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