TSC2 [p Ser939] Antibody (JE54-06)

Novus Biologicals | Catalog # NBP3-32812

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JE54-06 expressed in HEK293
View all TSC2 Primary Antibodies »

Product Specifications

Immunogen

Synthetic phosphopeptide corresponding to residues around Ser939 of human TSC2. (Uniprot: P49815)

Modification

p Ser 939

Localization

Cytoplasm, Membrane.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

201 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit TSC2 [p Ser939] Antibody (JE54-06) (NBP3-32812) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for TSC2 [p Ser939] Antibody (JE54-06)

TSC2 [p Ser939] Antibody (JE54-06)

Western Blot: TSC2 [p Ser939] Antibody (JE54-06) [NBP3-32812]

Western Blot: TSC2 [p Ser939] Antibody (JE54-06) [NBP3-32812] - Western blot analysis of TSC2 on different lysates with Rabbit anti-TSC2 antibody (NBP3-32812) at 1/1,000 dilution.

Lane 1: SH-SY5Y cell lysate
Lane 2: SH-SY5Y treated with 200nM Calyculin A and 1uM Okadaic Acid for 1 hour cell lysate
Lane 3: SH-SY5Y treated with 200nM Calyculin A and 1uM Okadaic Acid for 1 hour, then treated with pp for 1 hour cell lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 201 kDa
Observed band size: 250 kDa

Exposure time: 3 minutes;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32812) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Applications for TSC2 [p Ser939] Antibody (JE54-06)

Application
Recommended Usage

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

1*TBS (pH7.4), 0.05% BSA and 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: TSC2

Tuberous sclerosis complex-2 (TCS2 also known as Tuberin) is a tumor suppressor that forms a complex with TSC1 (Hamartin ) and this complex is known to control various cellular functions including cell cycle, endocytosis, adhesion, and transcription (1). The C-terminal region of TSC2 contains a GTPase-activating protein (GAP) domain which interacts with Rap1, Rab5 and Rheb (2). The TSC1/TSC2 complex inhibits phosphorylation of S6kinase and 4E-BP1 through inactivation of mTOR (3). Additionally, binding of 14-3-3 beta to TSC2 at Ser1210 reduce the ability of the complex to inhibit phosphorylation of S6 kinase (4). Phosphorylation by ATK at Ser924 and Thr1518 inactivates TSC2 and disrupts its interaction with TSC1 (5). Tuberous sclerosis (TSC), an autosomal dominant disorder that affects 1 in 6000 individuals, is caused by a mutation in either the TSC1 or TSC2 tumor suppressor gene.

Long Name

Tuberous Sclerosis 2

Alternate Names

LAM, TSC4, Tuberin

Gene Symbol

TSC2

Additional TSC2 Products

Product Documents for TSC2 [p Ser939] Antibody (JE54-06)

Certificate of Analysis

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Download SDS

Product Specific Notices for TSC2 [p Ser939] Antibody (JE54-06)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Interferons

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