TTF-1 / NKX2-1 Antibody (MSVA-312R)
Novus Biologicals | Catalog # NBP3-57273
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Rabbit IgG Clone # MSVA-312R
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Product Specifications
Immunogen
Recombinant human TTF-1 protein Swissprot P43699
Localization
Nucleus
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
This antibody is highly specific and optimized for use in Immunohistochemistry.
It has been validated on TMAs covering 76 normal tissues and numerous cancer tissues.
The antibody concentration is optimized for dilution within dilution range using commercially available antibody diluent for immunohistochemistry.
Positive Control
Postive control:Lung: Nuclear TTF1 staining should be strong in pneumocytes and in basal cells of the terminal bronchioles while a weaker, still moderate to strong staining should be seen in columnar epithelial cells of the terminal bronchioles (low expressor control)..
Negative Control
Negitve control:Liver: TTF1 staining must not be seen in any cells.
Storage
Antibody can be stored at 4degC. For extended storage, aliquot and store at -20degC. Avoid repeated freeze-thaw cycles.
Scientific Data Images for TTF-1 / NKX2-1 Antibody (MSVA-312R)
Immunohistochemistry-Paraffin:TTF-1 / NKX2-1 Antibody (MSVA-312R)NBP3-57273
Pulmonary adenocarcinoma with strong TTF1 staining of all tumor cells
Immunohistochemistry-Paraffin:TTF-1 / NKX2-1 Antibody (MSVA-312R)NBP3-57273
Thyroid gland with strong TTF1 staining of follicular epithelial cells
Immunohistochemistry-Paraffin:TTF-1 / NKX2-1 Antibody (MSVA-312R)NBP3-57273
Intense TTF1 positivity of pneumocytes
TTF-1 / NKX2-1 Cancer Tissue Gallery
TTF-1 / NKX2-1 Cancer Tissue Gallery
TTF-1 / NKX2-1 Normal Tissue Gallery
TTF-1 / NKX2-1 Normal Tissue Gallery
TTF-1 / NKX2-1 Normal Tissue Gallery
TTF-1 / NKX2-1 Normal Tissue Gallery
TTF-1 / NKX2-1 Normal Tissue Gallery
TTF-1 / NKX2-1 Normal Tissue Gallery
TTF-1 / NKX2-1 Normal Tissue Gallery
Applications for TTF-1 / NKX2-1 Antibody (MSVA-312R)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:100 1:200
Application Notes
Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:100 at 37C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS
Preservative
<0.1% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: TTF-1/NKX2-1
Long Name
NK2 Homeobox 1
Alternate Names
BCH, BHC, NKX2-1, NKX2.1, NKX2A, T/EBP, TITF1, TTF1
Gene Symbol
NKX2-1
Additional TTF-1/NKX2-1 Products
Product Documents for TTF-1 / NKX2-1 Antibody (MSVA-312R)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for TTF-1 / NKX2-1 Antibody (MSVA-312R)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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