TTF2 Antibody - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-98952
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
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Product Specifications
Immunogen
Produced in rabbits immunized with a synthetic peptide corresponding to the N-terminus of the Human TTF2.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for TTF2 Antibody - Azide and BSA Free
Western Blot: TTF2 Antibody [NBP2-98952]
Western Blot: TTF2 Antibody [NBP2-98952] - Anti-TTF2 rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 130 kDa. Observed band size: 145 kDaImmunoprecipitation: TTF2 Antibody [NBP2-98952]
Immunoprecipitation: TTF2 Antibody [NBP2-98952] - TTF2 was immunoprecipitated using: Lane A: 0.5 mg Hela Whole Cell Lysate1 ul anti-TTF2 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-TTF2 rabbit polyclonal antibody, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 127 kDa. Observed band size: 127 kDaApplications for TTF2 Antibody - Azide and BSA Free
Application
Recommended Usage
Immunoprecipitation
1-2 uL/mg of lysate
Western Blot
1:500-1:1000
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
0.2 um filtered solution in PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: TTF2
Alternate Names
EC 3.6.1, EC 3.6.4.-, F2, HuF2lodestar protein, human factor 2, Lodestar homolog, RNA polymerase II termination factor, Transcription release factor 2, transcription termination factor 2, transcription termination factor, RNA polymerase II
Gene Symbol
TTF2
Additional TTF2 Products
Product Documents for TTF2 Antibody - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for TTF2 Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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