Human Tuberculosis Antibody (IgG) ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP2-60187

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum (50 - 100 uL)
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Product Specifications

Assay Type

Indirect ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Human

Specificity

This assay has high sensitivity and excellent specificity for detection of human tuberculosis (TB) antibody (IgG). No significant cross-reactivity or interference between human tuberculosis (TB) antibody (IgG) and analogues was observed.

Description

Assay Length: 1-5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV 15

Inter-Assay Precision (Precision between assays) %CV 15

Kit Contents for Human Tuberculosis Antibody (IgG) ELISA Kit (Colorimetric)

  • Adhesive Strip (For 96 wells)
  • Coated assay plate
  • HRP-conjugate
  • Instruction manual
  • Negative Control
  • Positive Control
  • Sample Diluent
  • Stop Solution
  • Substrate A
  • Substrate B
  • Wash Buffer (25 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Tuberculosis Antibody (IgG)

This assay employs the qualitative enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with antigen. Samples are pipetted into the wells with anti-human IgG conjugated Horseradish Peroxidase (HRP). Any antibodies specific for the antigen present will bind to the pre-coated antigen. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of human tuberculosis (TB) antibody (IgG) bound in the initial step. The color development is stopped and the intensity of the color is measured.

Additional Tuberculosis Antibody (IgG) Products

Product Documents for Human Tuberculosis Antibody (IgG) ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Human Tuberculosis Antibody (IgG) ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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