TWEAK/TNFSF12 Antibody (MTW-1) - Chimeric - Azide and BSA Free

This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG1 format, for improved compatibility with existing reagents, assays and techniques.

The specificity of this antibody has been confirmed in flow cytometric analysis, where it reacts specifically with mTWEAK-transfected L5178Y cells, and not with parental L5178Y cells or cells transfected with related TNF family members (Nakayama et al, 2003). Additionally, this antibody has been used to assess TWEAK cell surface expression in flow cytometric analysis (Nakayama et al, 2003). This antibody has been shown to block CD8-mTWEAK cytotoxicity against L5178Y cells over-expressing murine Fn14, the TWEAK receptor (Nakayama et al, 2003). When administered to collagen-induced arthritis (CIA) mice, this blocking antibody has been shown to have anti-inflammatory and antiangiogenic effects, with associated improvements to both the clinical and histological manifestations of CIA (Kamata et al, 2006). Additionally, administration of this antibody to denervated mice attenuates denervation-induced skeletal muscle loss (Mittal et al, 2010). Finally, administration of this antibody to CD163-/- mice subject to hind limb ischaemia inhibits the increased perfusion resulting from CD163 deficiency (Akahori et al, 2015).