UBE2E3 Antibody (OTI1B4)

Novus Biologicals | Catalog # NBP2-46271

Novus Biologicals
Discontinued Product
NBP2-46271 has been discontinued. View all UBE2E3 products.

Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI1B4
View all UBE2E3 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human UBE2E3(NP_006348) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

22.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for UBE2E3 Antibody (OTI1B4)

Western Blot: UBE2E3 Antibody (OTI1B4) [NBP2-46271]

Western Blot: UBE2E3 Antibody (OTI1B4) [NBP2-46271]

Western Blot: UBE2E3 Antibody (1B4) [NBP2-46271] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UBE2E3.
Immunohistochemistry: UBE2E3 Antibody (OTI1B4) [NBP2-46271]

Immunohistochemistry: UBE2E3 Antibody (OTI1B4) [NBP2-46271]

Immunohistochemistry: UBE2E3 Antibody (1B4) [NBP2-46271] - Analysis of Adenocarcinoma of Human endometrium tissue.
Flow Cytometry: UBE2E3 Antibody (OTI1B4) [NBP2-46271]

Flow Cytometry: UBE2E3 Antibody (OTI1B4) [NBP2-46271]

Flow Cytometry: UBE2E3 Antibody (1B4) [NBP2-46271] - Analysis of HEK293T cells transfected with either overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by UBE2E3 antibody.
Western Blot: UBE2E3 Antibody (OTI1B4) [NBP2-46271]

Western Blot: UBE2E3 Antibody (OTI1B4) [NBP2-46271]

Western Blot: UBE2E3 Antibody (1B4) [NBP2-46271] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemistry: UBE2E3 Antibody (OTI1B4) [NBP2-46271]

Immunohistochemistry: UBE2E3 Antibody (OTI1B4) [NBP2-46271]

Immunohistochemistry: UBE2E3 Antibody (1B4) [NBP2-46271] - Analysis of Carcinoma of Human thyroid tissue.
Immunohistochemistry: UBE2E3 Antibody (OTI1B4) [NBP2-46271]

Immunohistochemistry: UBE2E3 Antibody (OTI1B4) [NBP2-46271]

Immunohistochemistry: UBE2E3 Antibody (1B4) [NBP2-46271] - Analysis of Human endometrium tissue.
Immunohistochemistry: UBE2E3 Antibody (OTI1B4) [NBP2-46271]

Immunohistochemistry: UBE2E3 Antibody (OTI1B4) [NBP2-46271]

Immunohistochemistry: UBE2E3 Antibody (1B4) [NBP2-46271] - Analysis of Carcinoma of Human lung tissue.
Immunohistochemistry: UBE2E3 Antibody (OTI1B4) [NBP2-46271]

Immunohistochemistry: UBE2E3 Antibody (OTI1B4) [NBP2-46271]

Immunohistochemistry: UBE2E3 Antibody (1B4) [NBP2-46271] - Analysis of Adenocarcinoma of Human ovary tissue.

Applications for UBE2E3 Antibody (OTI1B4)

Application
Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:150

Western Blot

1:500-2000

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: UBE2E3

Catalyzes the covalent attachment of ubiquitin to other proteins. Participates in the regulation of transepithelial sodium transport in renal cells. May be involved in cell growth arrest.

Alternate Names

E2-23K, UBCE4, UbcH9, UbcM2, UBE2E3

Gene Symbol

UBE2E3

Additional UBE2E3 Products

Product Documents for UBE2E3 Antibody (OTI1B4)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for UBE2E3 Antibody (OTI1B4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for UBE2E3 Antibody (OTI1B4)

There are currently no reviews for this product. Be the first to review UBE2E3 Antibody (OTI1B4) and earn rewards!

Have you used UBE2E3 Antibody (OTI1B4)?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies

Associated Pathways

Ubiquitination Cascade Pathway Ubiquitination Cascade Pathway Thumbnail