UBE2E3 Antibody (OTI1B4) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-74737

Novus Biologicals
Discontinued Product
NBP2-74737 has been discontinued. View all UBE2E3 products.

Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI1B4

Format

Azide and BSA Free
View all UBE2E3 Primary Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human UBE2E3(NP_006348) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

22.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for UBE2E3 Antibody (OTI1B4) - Azide and BSA Free

Western Blot: UBE2E3 Antibody (OTI1B4)Azide and BSA Free [NBP2-74737]

Western Blot: UBE2E3 Antibody (OTI1B4)Azide and BSA Free [NBP2-74737]

Western Blot: UBE2E3 Antibody (OTI1B4) - Azide and BSA Free [NBP2-74737] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY UBE2E3.
Immunohistochemistry: UBE2E3 Antibody (OTI1B4) - Azide and BSA Free [NBP2-74737]

Immunohistochemistry: UBE2E3 Antibody (OTI1B4) - Azide and BSA Free [NBP2-74737]

Immunohistochemistry: UBE2E3 Antibody (OTI1B4) - Azide and BSA Free [NBP2-74737] - Analysis of Carcinoma of Human thyroid tissue.
Flow Cytometry: UBE2E3 Antibody (OTI1B4) - Azide and BSA Free [NBP2-74737]

Flow Cytometry: UBE2E3 Antibody (OTI1B4) - Azide and BSA Free [NBP2-74737]

Flow Cytometry: UBE2E3 Antibody (OTI1B4) - Azide and BSA Free [NBP2-74737] - Analysis of HEK293T cells transfected with either overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostaining by UBE2E3 antibody.
Western Blot: UBE2E3 Antibody (OTI1B4)Azide and BSA Free [NBP2-74737]

Western Blot: UBE2E3 Antibody (OTI1B4)Azide and BSA Free [NBP2-74737]

Western Blot: UBE2E3 Antibody (OTI1B4) - Azide and BSA Free [NBP2-74737] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunohistochemistry: UBE2E3 Antibody (OTI1B4) - Azide and BSA Free [NBP2-74737]

Immunohistochemistry: UBE2E3 Antibody (OTI1B4) - Azide and BSA Free [NBP2-74737]

Immunohistochemistry: UBE2E3 Antibody (OTI1B4) - Azide and BSA Free [NBP2-74737] - Analysis of Adenocarcinoma of Human endometrium tissue.
Immunohistochemistry: UBE2E3 Antibody (OTI1B4) - Azide and BSA Free [NBP2-74737]

Immunohistochemistry: UBE2E3 Antibody (OTI1B4) - Azide and BSA Free [NBP2-74737]

Immunohistochemistry: UBE2E3 Antibody (OTI1B4) - Azide and BSA Free [NBP2-74737] - Analysis of Adenocarcinoma of Human ovary tissue.
Immunohistochemistry: UBE2E3 Antibody (OTI1B4) - Azide and BSA Free [NBP2-74737]

Immunohistochemistry: UBE2E3 Antibody (OTI1B4) - Azide and BSA Free [NBP2-74737]

Immunohistochemistry: UBE2E3 Antibody (OTI1B4) - Azide and BSA Free [NBP2-74737] - Analysis of Carcinoma of Human lung tissue.
Immunohistochemistry: UBE2E3 Antibody (OTI1B4) - Azide and BSA Free [NBP2-74737]

Immunohistochemistry: UBE2E3 Antibody (OTI1B4) - Azide and BSA Free [NBP2-74737]

Immunohistochemistry: UBE2E3 Antibody (OTI1B4) - Azide and BSA Free [NBP2-74737] - Analysis of Human endometrium tissue.

Applications for UBE2E3 Antibody (OTI1B4) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunohistochemistry

1:150

Western Blot

1:500-2000

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: UBE2E3

Catalyzes the covalent attachment of ubiquitin to other proteins. Participates in the regulation of transepithelial sodium transport in renal cells. May be involved in cell growth arrest.

Alternate Names

E2-23K, UBCE4, UbcH9, UbcM2, UBE2E3

Gene Symbol

UBE2E3

Additional UBE2E3 Products

Product Documents for UBE2E3 Antibody (OTI1B4) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for UBE2E3 Antibody (OTI1B4) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

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Associated Pathways

Ubiquitination Cascade Pathway Ubiquitination Cascade Pathway Thumbnail