UBE2F/NCE2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-19292

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Monkey (100%). Backed by our 100% Guarantee.

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all UBE2F Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human UBE2F/NCE2. The exact sequence is proprietary.

Reactivity Notes

Rhesus Monkey (100%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for UBE2F/NCE2 Antibody - BSA Free

Western Blot: UBE2F/NCE2 Antibody [NBP2-19292]

Western Blot: UBE2F/NCE2 Antibody [NBP2-19292]

Western Blot: UBE2F/NCE2 Antibody [NBP2-19292] - Analysis of UBE2F expression in transfected 293T cell line by UBE2F polyclonal antibody. A: Non-transfected lysate. B: UBE2F transfected lysate. 12% SDS PAGE gel, diluted at 1:500.

Applications for UBE2F/NCE2 Antibody - BSA Free

Application
Recommended Usage

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: UBE2F

Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5.

Long Name

Ubiquitin-conjugating Enzyme E2F

Alternate Names

NCE2

Gene Symbol

UBE2F

UniProt

Q969M7 (Human)

Additional UBE2F Products

Product Documents for UBE2F/NCE2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for UBE2F/NCE2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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FAQs

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Associated Pathways

Ubiquitination Cascade Pathway Ubiquitination Cascade Pathway Thumbnail